推拿手法对家兔骨骼肌失神经支配后成肌调节因子Myf-5、Myogenin表达的影响  

Effect of Massage Manipulation(Tuina) on Expression of Myogenic Regulatory Factors Myf-5 and Myogenin in Rabbit Skeletal Muscle after Denervation

作　　者：卢裕强 郭汝宝[2] LU Yuqiang;GUO Rubao(Department of Neurology,Tongde Hospital of Zhejiang Province,Hangzhou,Zhejiang province,310012,China;Department of Intervertebral Disc Medicine,Third Affiliated Hospital,Zhejiang Chinese Medicine University,Hangzhou,Zhejiang province,310005,China)

机构地区：[1]浙江省立同德医院神经内科,杭州310012 [2]浙江中医药大学附属第三医院椎间盘科,杭州310005

出　　处：《浙江中西医结合杂志》2019年第12期979-984,共6页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金项目(No.81303064,No.81873391)

摘　　要：目的观察推拿手法对家兔失神经支配骨骼肌干预后肌湿重、成肌调节因子中生肌因子5(Myogenic factor 5,Myf-5)、肌细胞生成素(Myogenin)表达的影响。方法普通级新西兰雄性家兔随机分为正常对照组、模型对照组、神经生长因子(nerve growth factor,NGF)治疗组和手法治疗组,每组30只。每组再随机分为2周、3周、1个月、2个月和4个月五个亚组,每组6只。模型对照组、NGF治疗组及手法治疗组家兔建立腓肠肌失神经支配模型,NGF治疗组给予肌注注射用鼠神经生长因子,手法干预组进行推拿干预,模型对照组及正常对照组不进行其他操作。分别干预后处死取材,称量腓肠肌肌湿重,计算术侧与健侧的腓肠肌肌湿重比值;检测腓肠肌组织Myf-5 mRNA、Myogenin mRNA表达水平。结果(1)腓肠肌肌湿重比:NGF治疗组在2周(0.79±0.03)%、3周(0.70±0.06)%、1个月(0.58±0.04)%、2个月(0.44±0.05)%,手法治疗组在2周(0.72±0.05)%、3周(0.55±0.04)%、1个月(0.55±0.09)%、2个月(0.49±0.03)%、4个月(0.50±0.03)%高于模型对照组同期[2周(0.53±0.01)%、3周(0.41±0.03)%、1个月(0.40±0.03)%、2个月(0.38±0.05)%、4个月(0.38±0.05)%,P<0.05]。手法治疗组在2周(0.72±0.05)%、3周(0.55±0.04)%低于NGF治疗组[2周(0.79±0.03)%、3周(0.70±0.06)%],4个月(0.50±0.03)%时则高于NGF治疗组(0.42±0.02)%(P<0.05)。(2)腓肠肌Myf-5 mRNA表达:NGF治疗组在2周(18.50±1.79)、3周(12.52±0.58)、1个月(5.30±1.76)、2个月(2.82±0.23),手法治疗组在2周(20.48±2.20)低于模型对照组同期[2周(40.91±10.31)、3周(25.25±1.27)、1个月(13.91±1.40)、2个月(8.83±0.86),P<0.05],手法治疗组在1个月(16.50±1.17)、2个月(12.20±0.84)、4个月(5.32±0.60)高于模型对照组[1个月(13.91±1.40)、2个月(8.83±0.86)、4个月(3.82±0.54),P<0.05]。手法治疗组在3周(37.21±9.10)、1个月(16.50±1.17)、2个月(12.20±0.84)、4个月(5.32±0.60)时高于NGF治疗组[3周(12.52±0.58)、1个月(5.30±1.76Objective To observe the effect of massage manipulation(Tuina)on muscle wet weight,expression of myogenic regulatory factors,i.e.myogenic factor 5(Myf-5)and myogenin,in rabbit skeletal muscle after denervation.Methods New Zealand male rabbits were randomly divided into normal control group,model control group,nerve growth factor(NGF)treatment group and Tuina treatment group,with 30 animals in each group.Then each group was randomly divided into 5 subgroups of 2 weeks,3 weeks,1 month,2 months and 4 months,with 6 rabbits in each group.Rabbits in model control group,NGF treatment group and Tuina treatment group were constructed as the gastrocnemius denervation model,then NGF treatment group was given intramuscular injection of rat NGF,Tuina group was given Tuina intervention,model control group and normal control group were not given any operations.After the intervention,rabbits were sacrificed,wet weight of gastrocnemius muscle was measured,and wet weight ratio between the operation side and the healthy side was calculated;the expression levels of Myf-5 and myogenin mRNA in gastrocnemius muscle were detected.Results(1)The gastrocnemius wet weight ratio in NGF group were(0.79±0.03)%at 2 weeks,(0.70±0.06)%at 3 weeks,(0.58±0.04)%at 1 month,and(0.44±0.05)at 2 months;in Tuina treatment group,(0.72±0.05)%at 2-w,(0.55±0.04)%at 3-w,(0.55±0.09)%at 1-mon,(0.49±0.03)%at 2-mon,(0.50±0.03)%at 4-mon,significantly higher than those of the model group[(0.53±0.01)%,(0.41±0.03)%,(0.40±0.03)%,(0.38±0.05)%and(0.38±0.05)%,respectively.P<0.05].Compare to the NGF group,Wet weight ratio of 2-w and 3-w in Tuina treatment group decreased,but increased at 4-mon[(0.72±0.05)%vs(0.79±0.03)%,(0.55±0.04)%vs(0.70±0.06)%,(0.50±0.03)%vs(0.42±0.02)%,P<0.05].(2)The mRNA expression level of Myf-5 in NGF group were(18.50±1.79)at 2-w,(12.52±0.58)at 3-w,(5.30±1.76)at 1-mon,and(2.82±0.23)at 2-mon;in Tuina treatment group at 2-w[(20.48±2.20)],it was lower than those of the model group[(40.91±10.31)at 2-w,(25.25±1.27)at 3-w,(13.91�

关 键 词：家兔 骨骼肌失神经支配 推拿手法 肌湿重比 成肌调节因子 

分 类 号：R74[医药卫生—神经病学与精神病学]