Vav1-Cre介导的YFP报告小鼠有效标记多组织器官中的巨噬细胞  

Vav1Cre strain efficiently recombined in macrophages of some tissues and organs when crossed with a YFP reporter mice

作　　者：李斯特 唐丽 LI Si-te;TANG Li(Institute of Lifeomics,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 102206,China;State Key Laboratory of Proteomics,Beijing Proteome Research Center,National Center for Protein Sciences(Beijing),Beijing 102206,China)

机构地区：[1]军事科学院军事医学研究院生命组学研究所,北京102206 [2]蛋白质组学国家重点实验室北京蛋白质组研究中心国家蛋白质科学中心(北京),北京102206

出　　处：《军事医学》2019年第6期421-425,430,共6页Military Medical Sciences

基　　金：国家自然科学基金（91842101）

摘　　要：目的利用Vav1-Cre介导的ROSA26R^YFP小鼠产生黄色荧光蛋白(YFP)的特性,检测其在不同器官中巨噬细胞的标记效率。方法通过基因型鉴定方法筛选出Vav1^Cre小鼠与ROSA26R^YFP小鼠杂交产生的双阳性子代小鼠,流式细胞术检测小鼠肝、脾、肾和肺中巨噬细胞的标记效率。结果Vav1^Cre与ROSA26R^YFP小鼠的杂交后代共19只,其中双阳性基因型小鼠(Vav1^CreROSA26R^YFP)9只;流式细胞术分析肝、脾、肾和肺中巨噬细胞YFP阳性细胞百分比(%)分别为91.49±1.332,98.02±0.3023,94.01±0.8127,91.39±1.47。结论Vav1^CreROSA26R^YFP小鼠能高效标记各组织器官中的巨噬细胞。Objective To detect the labeling efficiency of yellow fluorescent protein(YFP)in macrophages in different organs by using the characteristics of green fluorescent protein(GFP)produced by Vav1-Cre mediated ROSA26R^YFP mice.Methods Vav1^Cre mice and ROSA26R^YFP mice were identified by genotyping,and the YFP labeling efficiency of macrophages in the liver,spleen,kidney and lung were detected by flow cytometry.Results The percentage of YFP positive cells in the liver,spleen,kidney and lung macrophages by flow cytometry was 91.49±1.332,98.02±0.3023,94.01±0.8127 and 91.39±1.47,respectively.Conclusion Vav1^Cre strain can be efficiently recombined in macrophages(>90%)when crossing with a conditional reporter stain expressing YFP from the ubiquitously active Rosa26 locus(ROSA26RYFP).

关 键 词：巨噬细胞 报告小鼠 免疫系统 杂交 荧光蛋白 CRE重组酶 基因型 小鼠 基因敲除 小鼠 转基因 

分 类 号：R392-33[医药卫生—免疫学] R329.2[医药卫生—基础医学]