空气暴露及抗氧化剂对鲤血清生化、肌肉抗氧化及品质的影响  

Effects of air exposure and antioxidants on serum biochemical profiles, and antioxidant ability and quality of muscle of Songpu mirror carp Cyprinus carpio Songpu

作　　者：王连生[1] 李晋南[1] 吴迪 范泽 王常安[1] 赵志刚[1] 罗亮[1] 徐奇友[1,2] WANG Lian-sheng;LI Jin-nan;WU Di;FAN Ze;WANG Chang-an;ZHAO Zhi-gang;LUO Liang;XU Qi-you(Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;College of Life Science,Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development,Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition,Chinese Academy of Fishery Sciences,Huzhou University,Huzhou 313000,China)

机构地区：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070 [2]湖州师范学院生命科学学院,浙江省水生生物资源养护与开发技术研究重点实验室,中国水产科学研究院水生动物繁育与营养重点实验室,湖州313000

出　　处：《大连海洋大学学报》2019年第6期785-791,共7页Journal of Dalian Ocean University

基　　金：黑龙江水产研究所基本科研业务费专项(HSY201706M);国家大宗淡水鱼产业技术体系项目(CARS-45);国家自然科学基金资助项目(31802305);黑龙江省应用技术研究与开发计划项目(GA18B202)

摘　　要：为了研究空气暴露及抗氧化剂对松浦鲤Cyprinus carpio Songpu血清生化、肌肉抗氧化及品质的影响,试验设置5个组,分别饲喂基础饲料中添加600 mg/kg维生素E(VE组)、500 mg/kgγ-氨基丁酸(GABA组)、0.5 mg/kg亚硒酸钠(Se组)、600 mg/kg VE+500 mg/kg GABA+0.5 mg/kg亚硒酸钠(复合组)的试验饲料及不添加抗氧化剂的基础饲料(对照组),每组设3个重复,每个重复放15尾,共225尾鱼,鱼体质量为(1060±40)g,试验共进行13 d,养殖试验结束后饥饿24 h,检测空气暴露前和经空气暴露15 min后各组松浦镜鲤血清生化、肌肉抗氧化及品质相关指标。结果表明:空气暴露前,与对照组相比,饲料中添加各种抗氧化剂对松浦镜鲤血清生化指标无显著性影响(P>0.05),但复合抗氧化剂可显著提高鲤肌肉过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性,同时显著降低丙二醛(MDA)含量(P<0.05),Se可显著降低滴水损失(P<0.05);空气暴露15 min后,与对照组相比,饲料中添加GABA、VE和复合抗氧化剂可显著降低鲤血清皮质醇及葡萄糖含量(P<0.05),VE和复合抗氧化剂可显著提高鲤肌肉SOD、CAT活性并显著降低MDA含量(P<0.05),Se和复合抗氧化剂可显著提高谷胱甘肽(GSH)含量(P<0.05),VE及复合抗氧化剂显著降低鲤肌肉滴水损失,GABA和复合抗氧化剂显著降低鲤肌肉储存损失(P<0.05)。研究表明,VE和复合抗氧化剂均可缓解空气暴露对松浦镜鲤产生的应激胁迫,提高松浦镜鲤的肌肉抗氧化能力,并在一定程度上改善鲤的肌肉品质。Songpu mirror carp Cyprinus carpio Songpu weighting about(1060±40)g were reared in a 1.8 m×1.8 m×1.0 m net cage disposed in a recirculating tank and fed basic diet(control group),and the basic diet containing 600 mg/kg of vitamin E(group VE),500 mg/kg of gamma amino butyric acid(group GABA),0.5 mg/kg of sodium selenite(group Se)and 600 mg/kg VE+500 mg/kg GABA+0.5 mg/kg sodium selenite(composite group)for 13 days.Then physiological and biochemical indicators in serum sampled from caudal vein,and antioxidant capacity and quality in muscle were determined in the fish whose muscle was exposed to air for 15 min at air temperature of 21℃ before and after the air exposure in order to investigate the effects of air exposure and antioxidants on serum biochemistry,muscle antioxidant and quality of Songpu mirror carp.The results showed that there were significantly higher activities of catalase(CAT)and superoxide dismutase(SOD)and lower content of malondialdehyde(MDA)in the Songpu mirror carp in composite group and drip loss in group Se(P<0.05),without significant difference in blood biochemistry in the fish fed the diets containing antioxidants before air exposure(P>0.05).The Songpu mirror carp fed the diets containing GABA,VE and composite antioxidants and exposed to air for 15 min had significantly lower contents of blood cortisol and glucose(P<0.05),with significantly higher activities of SOD and CAT and lower content of MDA in group VE and composite group(P<0.05).There were significantly higher content of glutathione(GSH)in group Se and composite group(P<0.05)and significantly lower storage loss in muscle in group VE and composite group(P<0.05),indicating that the GABA and composite antioxidants lead to significant decrease in storage loss in muscle(P<0.05).The findings indicated that both VE and composite antioxidants alleviated the stress caused by air exposure on Songpu mirror crap and improved the antioxidant capacity and the quality of carp to a certain extent.

关 键 词：松浦镜鲤 空气暴露 抗氧化剂 血清生化 肌肉品质 

分 类 号：S963.73[农业科学—水产养殖]