不同大豆蛋白源替代鱼粉对洛氏鱥生长、非特异性免疫及蛋白质代谢酶活力的影响  

Effects of replacing fish meal with different soybean proteins on growth performance, non-specific immune indices and protein metabolism in Rhynchocypris lagowskii Dybowski

作　　者：段晶 吴莉芳 王婧瑶 张东鸣 祖岫杰[2] 刘艳辉[2] 金昌洙 DUAN Jing;WU Li-fang;WANG Jing-yao;ZHANG Dong-ming;ZU Xiu-jie;LIU Yan-hui;JIN Chang-zhu(College of Animal Science and Technology,Key Laboratory of Animal Production,Product Quality and Security,Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science,Jilin Agricultural University,Changchun 130118,China;Fisheries Science Research Institute of Jilin Province,Changchun 130033,China;Jinyuan Aquatic Products Field of Changyi District Jilin City,Jilin 132001,China)

机构地区：[1]吉林农业大学动物科学技术学院,动物生产及产品质量安全教育部重点实验室,吉林省动物营养与饲料科学重点实验室,吉林长春130118 [2]吉林省水产科学研究院,吉林长春130033 [3]吉林市昌邑区金源水产良种场,吉林吉林132001

出　　处：《大连海洋大学学报》2019年第6期804-810,共7页Journal of Dalian Ocean University

基　　金：吉林省科技厅重点科技攻关项目(20160204019NY)

摘　　要：为研究不同大豆蛋白源[豆粕(SBM)、发酵豆粕(FSBM)、全脂豆粉(FFSBM)和膨化大豆粉(ESBM)]替代50%的鱼粉对洛氏鱥Rhynchocypris lagowskii Dybowski生长、非特异性免疫及蛋白质代谢酶活力的影响,采用体质量为(6.09±0.03)g的洛氏鱥幼鱼为研究对象,利用SBM、FSBM、FFSBM和ESBM分别替代50%的鱼粉蛋白,配制成5种等氮、等能的半精制饲料,试验设5组,每组设3个重复,每个重复放养40尾洛氏鱥,养殖试验周期为56 d。结果表明:SBM、FSBM、FFSBM和ESBM组洛氏鱥的终末体质量、体质量增加率、特定生长率均显著低于对照组(P<0.05),而FSBM和ESBM组的终末体质量、体质量增加率、特定生长率显著高于SBM和FFSBM组(P<0.05);FSBM组洛氏鱥肌肉中粗蛋白质含量与对照组无显著性差异(P>0.05),而SBM、FFSBM、ESBM组则显著低于对照组(P<0.05);SBM、FSBM、FFSBM和ESBM组洛氏鱥溶菌酶(LZM)活力显著低于对照组(P<0.05),FSBM组和ESBM组间无显著性差异(P>0.05),但二者均显著高于SBM组和FFSBM组(P<0.05);SBM、FSBM、SBM和ESBM组洛氏鱥肝胰脏、前肠、后肠蛋白酶活力显著低于对照组(P<0.05),SBM和FFSBM组中肠蛋白酶活力显著低于对照组(P<0.05);SBM、FSBM、FFSBM和ESBM组洛氏鱥肝胰脏中谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力显著低于对照组(P<0.05),FSBM组洛氏鱥GPT和GOT活力显著高于SBM、FFSBM(P<0.05)。研究表明,在洛氏鱥配合饲料中,FSBM替代鱼粉效果最好,ESBM次之,而FFSBM的效果最差。A 56-day feeding trial was conducted to evaluate the effects of replacing fish meal with different soybean proteins on growth performance,non-specific immune indices and protein metabolism of fish Rhynchocypris lagowskii Dybowski with initial body weight of(6.09±0.03)g.The fish juveniles were reared in a temperature control culture system and fed five isonitrogen and isoenergic diets containing normal amount of fish meal(control group),soybean meal(SBM group),fermented soybean meal(FSBM group),full fat soybean meal(FFSBM group),and expanded soybean powder(ESBM group)replacing 50%of fish meal with 3 replicates with 40 fish in each treatment at water temperature of 22-24℃.It was found that there were significantly lower final body weight(FBW),weight gain rate(WGR),and specific growth rate(SGR)in the fish in SBM group,FSBM group,FFSBM group,and ESBM group than those in the control group(P<0.05).However,FBW,WGR,and SGR were significantly higher in FSBM group,and ESBM group than those in SBM group and FFSBM group(P<0.05).The juveniles in FSBM group had crude protein content in muscles,without significantly different from that in the control group(P>0.05),while the fish juveniles in SBM,FFSBM and ESBM groups had significantly lower muscular crude protein content than the fish in the control group did(P<0.05).There was significantly lower activity of lysozyme(LZM)in SBM,FSBM,FFSBM and ESBM groups than that in the control group(P<0.05),without significant difference between FSBM and ESBM groups(P>0.05),and significantly higher than that in SBM and the FFSBM groups(P<0.05).Significantly lower protease activities in hepatopancreas,foregut,and hindgut were observed in the fish in SBM,FSBM,FFSBM,and ESBM groups than that in the control group(P<0.05).Protease activity of middle intestine were significantly lower in SBM and FFSBM groups than that in the control group(P<0.05).Glutamic oxaloacetic transaminase GOT and glutamic-pyruvic transaminase(GPT)activities were found to be significantly lower in SBM,FSBM,FFSBM,and ESBM

关 键 词：洛氏鱥 大豆蛋白源 生长 非特异性免疫 蛋白质代谢酶 

分 类 号：S963[农业科学—水产养殖]