艽龙胶囊及其主成分对功能性消化不良大鼠的治疗作用  

Therapeutic effect and mechanism of Jiaolong capsule and its main components on rats with functional dyspepsia

作　　者：郭伦锋 刘铁明 袁鹰 董明芝 谢艳华 王四旺 Guo Lunfeng;Liu Tieming;Yuan Ying;Dong Mingzhi;Xie Yanhua;Wang Siwang(Ankang City Center Hospital Pharmacy,Ankang,725000,China;Xi'an Zhengda Pharmaceutical Co.,Ltd.,Xi’an,710043,China;The College of Life Sciences,Northwest University,Xi’an,710069,China)

机构地区：[1]安康市中心医院药剂科,陕西安康725000 [2]西安正大制药有限公司,陕西西安710043 [3]西北大学生命科学与医学部,陕西西安710069

出　　处：《贵州医药》2019年第11期1707-1710,共4页Guizhou Medical Journal

基　　金：陕西省创新药物研究中心(现代中药新药研发)平台建设(2015SF2-08-01);陕西省药用植物物效研究与新药创制工程技术研究中心(后补助课题:S2018-ZC-GCZXXY-SF-0005);陕西省生物医药重点实验室(后补助:2018SZS-41)课题资助

摘　　要：目的观察艽龙胶囊及其主成分对功能性消化不良大鼠的治疗作用。方法 80只SD大鼠,随机分为正常、模型、艽龙胶囊、龙胆苦苷、马钱苷酸、6′-O-β-D-葡萄糖基龙胆苦苷、山栀苷甲酯和多潘立酮组。造模后第1天,各试药组依次灌胃给予相应药物治疗,共4周。给药结束后,计算胃排空率和肠推进率,ELISA检测大鼠血清中胃饥饿素(GH)和血管活性肠肽(VIP)水平;Western blot检测胃窦组织中蛋白激酶样内质网激酶(PERK)和α-真核翻译起始因子2αβ-eIF2α蛋白表达。结果与正常组比较,模型组大鼠胃排空率、肠推进率及GH含量显著下降,而VIP、胃窦组织内PERK和p-eIF2α蛋白表达升高(P<0.01);经艽龙胶囊、龙胆苦苷和山栀苷甲酯治疗的大鼠胃排空率和肠推进率及GH含量明显升高,血清中VIP、胃窦组织内PERK和p-eIF2α蛋白表达显著降低(P<0.01)。结论艽龙胶囊能通过促进GH表达,抑制VIP、PERK和p-eIF2α蛋白表达,改善胃动力,提升胃肠功能,且其主要功效成分为龙胆苦苷和山栀苷甲酯。Objective To explore the therapeutic effect and mechanism of Jiaolong capsule and its main components on rats with functional dyspepsia. Method Eighty rats were randomly divided into normal, model, Jiaolong capsule, gentiopicroside, Loganic acid, 6’-O-β-D-glucosyl gentiopicroside, Shanzhiside methyl ester and domperidone Group, a total of 8 groups. On the 1 st day after modeling, each test group was administered with 44.00 mg/kg Jiaolong Capsule, 28.60 mg/kg gentiopicroside, 6.60 mg/kg of Loganic acid, 2.86 mg/kg of 6’-O-β-D-glucosyl gentiopicroside, 0.44 mg/kg Shanzhiside methyl ester and 2.70 mg/kg domperidone, once a day for 4 weeks. After the end of the administration, the gastric emptying rate and intestinal propulsion rate were calculated. The levels of ghrelin(GH) and vasoactive intestinal peptide(VIP) in the serum of rats were detected by ELISA. The expression of PERK and p-eIF2αprotein in gastric antrum were detected by Western blot. Results Compared with the normal group, the gastric emptying rate, intestinal propulsion rate and GH content of the model group were significantly decreased, while the expression of PERK and p-eIF2α protein in gastric antrum tissue and VIP in serum were increased(P<0.01). Gastric emptying rate, intestinal propulsion rate and GH content were significantly increased in rats treated with Jiaolong capsule, gentiopicroside and Shanzhiside methyl ester, and the expression of PERK and p-eIF2α protein and VIP content were significantly decreased(P<0.01). Conclusion Jiaolong capsule could improve gastric motility and enhance gastrointestinal function by promoting GH expression, inhibit the expression of VIP, PERK and p-eIF2α protein, and its main functional components may be gentiopicroside and Shanzhiside methyl ester.

关 键 词：功能性消化不良 艽龙胶囊 龙胆苦苷 山栀苷甲酯 

分 类 号：R573[医药卫生—消化系统] R322.44[医药卫生—内科学]