利用超高效液相色谱-飞行时间-串联质谱法分析青刺果油中的脂质成分  

Lipid of Prinsepia utilis Royle oil by utra-performance liquid chromatography-time of flight-tandem mass spectrometry

作　　者：卫海莲 吕昕[1] 谢亚[1] 徐淑玲 陈洪[1] 魏芳[1] WEI Hai-lian;LYU Xin;XIE Ya;XU Shu-ling;CHEN Hong;WEI Fang(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Oil Processing of Ministry of Agriculture and Rural Affairs,Hubei Key Laboratory of Lipid Chemistry and Nutrition,Oil Crops and Lipids Process Technology National&Local Joint Engineering Laboratory,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所农业农村部油料加工重点实验室油料脂质化学与营养湖北省重点实验室油料油脂加工技术国家地方联合工程实验室

出　　处：《中国油料作物学报》2019年第6期947-955,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31571926);湖北省技术创新项目(2018AHB014);武汉市科技计划项目(2019020701011468);生物医学分析化学教育部重点实验室(武汉大学)开放课题(ACBM2018002);中国农业科学院创新工程(CAAS-ASTIP-2013-OCRI)

摘　　要：青刺果为多年生稀有木本油料植物,是我国特有民族野生资源。在对青刺果油中脂肪酸组成分析的基础上,建立了超高效液相色谱-飞行时间-串联质谱的脂质分析方法,对其所含脂质的种类、组成、相对含量作分析。结果显示,在青刺果油主要脂肪酸的含量为:油酸39.17%、亚油酸36.16%、棕榈酸16.38%;从比例上看,饱和脂肪酸:单不饱和脂肪酸:多不饱和脂肪酸比例接近0.6:1:1。共鉴定出169种脂质,包括76种磷脂,84种甘油酯和9种糖脂;其中磷脂、甘油酯和糖脂的主要成分分别为磷脂酰肌醇、甘油三酯和双半乳糖甘油二酯。Prinsepia utilis Royleis a perennial rare woody oil plant, with high nutritional value and extensive pharmacological effect, which is unique national wild resources in China. Based on fatty acid composition analysis,a method of ultra-performance liquid chromatography-time of flight-tandem mass spectrometry(UPLC-TOF-MS/MS) was established and applied to analyze lipids types, composition and relative content in P. utilis Royle oil. Results showed that P. utilis Royle oil contained 39.17% oleic acid, 36.16% linoleic acid and 16.38% palmitic. The ratio of saturated fatty acid:monounsaturated fatty acid:polyunsaturated fatty acid was close to 0.6:1:1. Totally 169 lipids speices were found, including 76 phospholipids, 84 glycerides and 9 glycolipids. Among them, phosphatidylinositols and triacylglycerols were dominant phospholipids and glycerides respectively. Meanwhile, glycolipids mainly contained digalactosyldiacylglycerols. Ultra-performance liquid chromatography-time of flight-tandem mass spectrometry showed its advantages of high sensitivity, good accuracy and high throughput on plant lipid detection.

关 键 词：超高效液相色谱-飞行时间-串联质谱 木本油料 青刺果油 脂质 

分 类 号：S727.32[农业科学—林木遗传育种] O657.72[农业科学—林学]