中性蛋白酶催化水解污泥提取蛋白质的研究  

Study on protein extraction by sludge catalytic hydrolysis with neutral protease

作　　者：李超[1] 高健磊[2] 闫怡新[2] 岳秋彩 LI Chao;GAO Jianlei;YAN Yixin;YUE Qiucai(Tianjin Municipal Engineering Design&Research Institute Zhengzhou Branch,Zhengzhou 450001,China;School of water Conservancg and Engineering,Zhengzhou University,Zhengzhou 450001,China;Henan Xinshu Co.,Ltd.,Zhengzhou 450001,China)

机构地区：[1]天津市市政工程设计研究院郑州分院,河南郑州450001 [2]郑州大学水利科学与工程学院,河南郑州450001 [3]河南省鑫属实业有限公司,河南郑州450001

出　　处：《能源环境保护》2019年第6期18-22,共5页Energy Environmental Protection

摘　　要：为解决污泥二次污染问题并增加蛋白质来源,利用中性蛋白酶催化水解污泥提取蛋白质,分析了pH、酶浓度、反应时间、温度、底物含固率等因素对酶催化作用的影响,并确定酶作用时的最佳条件。结果表明:在pH=7.4、酶加量4000 U/g污泥、反应时间4 h、温度50℃、含固率4%的条件下,污泥蛋白质提取达到27.4%,氨基酸含量为373 mg/L,多肽含量为912 mg/L;利用蛋白酶作为污泥处理剂获取蛋白质、氨基酸和多肽,具有探索意义和应用价值。In order to solve secondary pollution of sludge and increase protein sources,sludge was catalytic hydrolyzed by neutral protease to extract protein.The effects of pH,enzyme concentration,reaction time,temperature and solids content ratio of substrate on catalysis were analyzed.The optimal conditions for catalysis were determined.The results showed that 27.4%of protein was extracted with 373 mg/L amino acid and 912 mg/L polypeptide,when pH=7.4,enzyme concentration=4000 U/g sludge,reaction time=4 h,temperature=50℃and solids content ratio=4%.Therefore,it has high theory exploration sense and application value to use protease as a sludge treatment agent to obtain protein,amino acids and polypeptides.

关 键 词：剩余污泥 中性蛋白酶 催化水解 蛋白质 

分 类 号：X705[环境科学与工程—环境工程]