Fas配体过表达对人牙周膜干细胞成骨分化的影响  

Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs

作　　者：马灵芝 任娅岚 钱丽萍 戈文斌 刘亚丽[1] 周治[2] MA Lingzhi;REN Yalan;QIAN Liping;GE Wenbin;LIU Yali;ZHOU Zhi(School of Stomatology,Kunming Medical University,Kunming 650500,China;Department of Orthodontics,The Second People's Hospital of Yunnan Province,Kunming 650021,China)

机构地区：[1]昆明医科大学口腔医学院,云南昆明650500 [2]云南省第二人民医院口腔正畸科,云南昆明650021

出　　处：《口腔医学研究》2019年第12期1169-1172,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:31301067、31860326);云南省应用基础研究计划项目(编号:2017FE468-227、2018FE001-260);昆明医科大学研究生创新基金(编号:2019S018)

摘　　要：目的:探讨Fas配体(Fas ligand,FasL)过表达对人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)骨向分化能力的影响。方法:体外有限稀释克隆化培养人PDLSCs,构建FasL过表达质粒,转染至PDLSCs,实验分为对照组(Control组)、空载体对照组(NC组)、FasL过表达组(EX组),对各组进行成骨诱导,茜素红染色、实时荧光定量PCR(Real-time PCR)和Western blot检测PDLSCs成骨分化的能力。结果:EX组成骨分化能力较Control组和NC组有明显升高(P<0.05),Control组和NC组比较差异无统计学意义(P>0.05)。结论:FasL可以促进PDLSCs成骨分化。Objective:To investigate the effect of FasL overexpression on osteogenic differentiation of human periodontal ligament stem cells(PDLSCs).Methods:Human PDLSCs were cloned and cultured in vitro with limited dilution.FasL overexpression plasmid was constructed and transfected into PDLSCs.The experiment was divided into control group,no-load control group(NC group),and FasL overexpression group(EX group).Osteogenic was induced in each group.Alizarin red staining,real-time PCR,and western blot were used to detect the osteogenic differentiation ability of PDLSCs.Results:The osteogenic differentiation ability of EX group was significantly higher than that of control group and NC group(P<0.05).There was no significant difference between control group and NC group(P>0.05).Conclusion:FasL can promote the osteogenic differentiation of human PDLSCs.

关 键 词：FAS配体 牙周膜干细胞 骨向分化 

分 类 号：R73[医药卫生—肿瘤]