低频磁刺激对高糖诱导人牙周膜细胞损伤的保护作用  

Protective Effect of Low-frequency Magnetic Stimulation on Human Periodontal Ligament Cells Injured by High Glucose

作　　者：郑广明 张健[2] 张文怡[3] ZHENG Guangming;ZHANG Jian;ZHANG Wenyi(Department of Stomatology,Tianjin Xianshui Hospital,Tianjin 300350,China;Department of Oral and Maxillofacial Surgery,Stomatological Hospital Affiliated to Tianjin Medical University,Tianjin 300070,China;Department of Prosthodontics,Stomatological Hospital Affiliated to Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津市津南医院口腔科,天津300350 [2]天津医科大学附属口腔医院口腔颌面外科,天津300070 [3]天津医科大学附属口腔医院修复科,天津300070

出　　处：《口腔医学研究》2019年第12期1177-1181,共5页Journal of Oral Science Research

摘　　要：目的:探讨低频磁刺激对高糖介导人牙周膜细胞损伤的保护作用。方法:体外复苏培养人牙周膜细胞,随机分为正常对照组和高糖模型组以及低频磁刺激组(3个亚组)共5个实验组。流式细胞术的方法检测细胞周期的变化,TUNEL法检测细胞的凋亡情况,比色法检测Caspase-3、6、9活性,逆转录PCR及Western-blot检测CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达水平。结果:与正常对照组比较,高糖模型中人牙周膜细胞凋亡增加;Caspase-3、6、9活性均得到了显著提高(P<0.05),CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达显著降低(P<0.05)。与高糖模型组比较,低频磁刺激组细胞凋亡率降低,Caspase-3、6、9活性降低(P<0.05),CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达上调(P<0.05);对细胞周期的影响不明显(P>0.05),3个亚组比较时,刺激强度为1.5 T时以上变化最明显(P<0.05)。结论:高糖条件促进人牙周膜细胞凋亡,低频磁刺激对高糖诱导人牙周膜细胞损伤有保护作用。Objective:To investigate the protective effect of low-frequency magnetic stimulation on periodontal membrane injury mediated by high sugar.Methods:Human periodontal membrane cells were cultured by resuscitation in vitro,which were randomly divided into normal control group,high sugar model group,and low-frequency magnetic stimulation group(3 subgroups).Flow cytometry method was used to detect the changes in cell cycle.TUNEL method was used to detect apoptosis.Colorimetric method was used to detect Caspase-3,6,and 9 activities,and reverse transcription PCR and Western-blot were used to detect CXCL1,CXCL2,and its receptor CXCR2 gene and protein expression levels.Results:Compared with the normal control group,the apoptosis of human periodontal membrane cells increased in the high sugar model.Caspase-3,6,and 9 activities were all improved(P<0.05).The expressions of CXCL1,CXCL2,and its receptor CXCR2 genes and proteins were significantly reduced(P<0.05).Compared with the high-sugar model group,the cell apoptosis rate in the low-frequency magnetic stimulation group decreased,and the Caspase-3,6,and 9 activities decreased(P<0.05).CXCL1,CXCL2,and its receptor CXCR2 gene and protein expression increased(P<0.05).The effect on the cell cycle was not obvious(P>0.05).When comparing three subgroups,the change in stimulus intensity above 1.5T was the most obvious(P<0.05).Conclusion:High sugar conditions lead to apoptosis of human periodontal membrane cells,and low-frequency magnetic stimulation has protective effect on human periodontal membrane cell injury induced by high sugar.

关 键 词：低频磁刺激 人牙周膜细胞 高糖 损伤 

分 类 号：R78[医药卫生—口腔医学]