口腔扁平苔藓患者唾液中氧化应激指标与NF-κB相关炎症因子相关性的研究  

Study on Relationship between Oxidative Stress and NF-κB-related Inflammatory Factors in Patients with Oral Lichen Planus

作　　者：李新[1] 孙银银 潘英潇 王赛男 张馨予[1] 卢恕来[3] LI Xin;SUN Yinyin;PAN Yingxiao;WANG Sainan;ZHANG Xinyu;LU Shulai(School of Stomatology,Qingdao University,Qingdao 266003,China;Dalian Medical University,Dalian 116027,China;Qingdao Municipal Hospital affiliated to Qingdao University,Qingdao 266011,China)

机构地区：[1]青岛大学口腔医学院,山东青岛266003 [2]大连医科大学,辽宁大连116027 [3]青岛大学附属青岛市市立医院,山东青岛266011

出　　处：《口腔医学研究》2019年第12期1187-1190,共4页Journal of Oral Science Research

基　　金：山东省医药卫生科技发展计划项目面上项目(2015WS0327);山东省卫计委中医药科技发展计划项目(2015-378);青岛市科技计划项目(17-3-3-39-nsh)

摘　　要：目的:通过唾液样本评估口腔扁平苔藓(oral lichen planus,OLP)患者氧化应激状态、NF-κB相关炎症因子水平及二者之间的相关性。方法:选取30例OLP患者(OLP组)和30名健康体检者(健康组),OLP组分为非糜烂型口腔扁平苔藓(non-erosive oral lichen planus,NEOLP)组和糜烂型口腔扁平苔藓(erosive oral lichen planus,EOLP)组,ELISA法检测氧化应激指标MDA、8-OHDG、TOS、TAC,NF-κB相关炎症因子TNF-α、IL-6、IL-8表达水平。结果:OLP组、NEOLP组、EOLP组MDA、8-OHDG、TOS均显著高于健康组(P<0.05),TAC显著降低(P<0.05)。OLP、NEOLP、EOLP组TNF-α、IL-6、IL-8显著高于健康组(P<0.05)。与NEOLP组比较,EOLP组中MDA、8-OHDG升高(P<0.05),TAC、TOS比较无差异(P>0.05);EOLP组TNF-α、IL-6升高,差异无统计学意义(P>0.05),IL-8显著升高(P<0.05)。OLP组、NEOLP组、EOLP组TOS与TNF-α、IL-6、IL-8呈正相关(P<0.05),TAC与TNF-α、IL-6、IL-8呈负相关(P<0.05)。结论:OLP患者存在氧化应激增加,NF-κB相关炎症因子水平增高,二者存在相关性。Objective:To evaluate the oxidative stress status and NF-κB-related inflammatory factors of oral lichen planus patients by saliva samples,and to explore the correlation of them.Methods:Thirty patients with oral lichen planus(the OLP group)and 30 healthy people(the healthy controls)were selected.The OLP group was divided into non-erosive oral lichen planus(NEOLP)group and erosive oral lichen planus(EOLP)group.Indicators of oxidative stress MDA,8-OHDG,TOS,TAC,and NF-κB related inflammatory factors IL-6,IL-8,and TNF-α were determined by using ELISA.Results:MDA,8-OHDG,and TOS of OLP group,NEOLP group,and EOLP group were significantly higher than those of healthy people(P<0.05).TAC was significantly lower(P<0.05).The levels of TNF-α,IL-6,and IL-8 in OLP group,NEOLP group,and EOLP group were significantly higher than those in healthy controls(P<0.05).Compared with NEOLP group,MDA and 8-OHDG in EOLP patients increased(P<0.05),while TOC and TOS had no significant difference(P>0.05).There was no significant difference in TNF-αand IL-6 between EOLP patients and EOLP patients(P>0.05),but IL-8 increased significantly(P<0.05).TOS was positively correlated with TNF-α,IL-6,and IL-8 in OLP group,NEOLP group,and EOLP group(P<0.05),while TAC was negatively correlated with TNF-α,IL-6,and IL-8(P<0.05).Conclusion:There was an increase in oxidative stress and the level of NF-κB related inflammatory factors in OLP patients,and there was a correlation between them.

关 键 词：口腔扁平苔藓 氧化应激 NF-ΚB 

分 类 号：R78[医药卫生—口腔医学]