质子泵在不同氮素形态调控茶树磷素吸收的功能研究  

The Role of Plasma Membrane H^+-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants

作　　者：陈林木 陈景光 王宁宁 张显晨[1] CHEN Linmu;CHEN Jingguang;WANG Ningning;ZHANG Xianchen(State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518116,China)

机构地区：[1]安徽农业大学茶树生物学及资源利用国家重点实验室,安徽合肥230036 [2]中国农业科学院深圳农业基因组研究所,广东深圳518116

出　　处：《茶叶科学》2019年第6期723-730,共8页Journal of Tea Science

基　　金：国家自然科学基金资助项目（341800583）;安徽省自然基金（KJ2017A126）

摘　　要：磷是植物生长发育的重要矿质营养元素之一,不同氮素形态均影响植物对磷素的吸收。植物细胞膜H^+-ATPase在矿质营养元素吸收过程中具有重要调控作用,因此不同氮素形态调控茶树磷素吸收可能与细胞膜H^+-ATPase相关。本研究采用二相分离法提取茶树根系质膜,并通过非损伤微测(NMT)、Western-blot等技术探究不同氮素形态对舒茶早根系磷素吸收和细胞膜H^+-ATPase特征参数的影响。结果表明,铵态氮提高茶树对磷素的吸收;其茶树根系细胞膜电位、H^+跨膜运输、H^+-ATPase活性和蛋白表达均高于硝态氮处理;且细胞膜H^+-ATPase专一抑制剂正钒酸钠(Na3VO4)显著减少不同氮素形态下茶树根系对磷素的吸收和富集。由此可见,茶树根系H^+-ATPase可能参与不同氮素形态调控磷素的吸收。Phosphorus is one of the most important mineral elements for plant growth and development,and different nitrogen forms can regulate phosphorus uptake.As is well-known that plant plasma membrane(PM)H^+-ATPase plays an important role in nutrition uptake,it may also relate to the different nitrogen-modulated phosphorus uptake.In our study,PM H^+-ATPase in tea roots were isolated by two-phase partitioning in aqueous dextranT-500 and polyethylene glycol,and the effect of different nitrogen forms on phosphorus uptake and the characteristic parameters of PM H^+-ATPase in tea roots were examined by using non-invasive micro-test technique and Western-blot.It was found that ammonium significantly improved the phosphorus accumulation in tea plants as compared with nitrate nutrition.In addition,less depolarized PM potentials,higher net H^+flux,PM H^+-ATPase activities and protein levels were found under ammonium treatment than those under nitrate nutrition treatment.Furthermore,vanadate(the PM H^+-ATPase inhibitor)significantly decreased phosphorus accumulation in tea plants under ammonium and nitrate nutrition treatments,which further suggests that PM H^+-ATPase may be involved in phosphorus accumulation in tea plants regulated by different nitrogen forms.

关 键 词：铵态氮 硝态氮 磷素吸收 细胞膜H^+-ATPase 茶树 

分 类 号：S571.1[农业科学—茶叶生产加工] S154.1[农业科学—作物学]