秦巴硒菇提取物FA-2-b-β诱导CD34^+CD38^--KG1a白血病干细胞凋亡及其相关机制  

Primary Mechanisms of CD34^+CD38^--KG1a Leukemia Stem Cells Apoptosis Induced by FA-2-b-β Extracted from Qinba Selenium-Mushroom

作　　者：王东萍[1] 石玮[2] 葛万文 张菊霞 赵莲萍[1] 陈雪 董莉[4] 孙延庆[1] WANG Dong-Ping;SHI Wei;GE Wan-Wen;ZHANG Ju-Xia;ZHAO Lian-Ping;CHEN Xue;DONG Li;SUN Yan-Qing(Department of Clinical Teaching,Gansu Provincial People's Hospital,Lanzhou 730000,Gansu Province,China;Department of Urology,The Second Hospital of Lanzhou University,Lanzhou 730030,Gansu Province,China;Department of Cuiying Biomedical Research Centery The Second Hospital of Lanzhou University,Lanzhou 730030,Gansu Province,China;Department of Central Gansu Provincial People's Hospital,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃省人民医院临床教学部,甘肃兰州730000 [2]兰州大学第二医院泌尿外科,甘肃兰州730030 [3]兰州大学第二医院萃英生物医学研究中心,甘肃兰州730030 [4]甘肃省人民医院中心实验室,甘肃兰州730000

出　　处：《中国实验血液学杂志》2019年第6期1761-1766,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(81560670);甘肃省青年科技基金计划(17JR5RA228);甘肃省自然科学基金(1606RJZA089),甘肃省自然科学基金(1506RJZA252)

摘　　要：目的:探讨秦巴硒菇提取物FA-2-b-β对CD34^+CD38^--KG1a白血病干细胞凋亡的诱导及其相关机制。方法:用免疫磁珠分选法从KG1a细胞株中分选出CD34^+CD38^--KG1a干细胞;不同浓度的FA-2-b-β(1.2-2.4mg/ml)体外干预KG1a干细胞24、48 h,应用CCK-8检测其增殖能力变化;流式细胞术检测FA-2-b-β干预KG1a干细胞后各组细胞凋亡比例;采用Western blot检测BAX、BCL-2、Casepase-3及Cyclin D1蛋白表达。结果:经过免疫磁珠分选后KG1a干细胞比例为(95.35±2.63)%。FA-2-b-β对KG1a干细胞的增殖具有明显抑制作用,呈时间和剂量依赖性(24 h,r=0.943;48 h,r=0.976)。流式细胞术检测显示,不同浓度FA-2-b-β作用KG1a干细胞24 h时,随药物浓度增加凋亡率也随之增高。Western blot检测显示,凋亡相关蛋白BAX和Casepase-3表达上调,BCL-2和Cyclin D1的表达下调。结论:秦巴硒菇提取物FA-2-b-β能够调控KG1a干细胞的增殖和凋亡,其抗肿瘤效应可能通过调控线粒体介导的凋亡途径实现。Objective:To investigate the apoptosis of CD34^+CD38^-KG1 a leukemia stem cells induced by Qinba selenium-mushroom extract(FA-2-b-β),and its related mechanism.Methods:CD34^+CD38~KG1 a cells were isolated from KGla cell line by magnetic activated cell sorting.The proliferation ability of KGla stem cells treatd by various concentration of FA-2-b-β(1.2-2.4 mg/ml)in vitro for 24 and 48 hours were tested by cell counting Kit-8(CCK8).Flow cytometry was used to detect the apoptosis rate of KGla stem cells in each group after treated by FA-2-b-βin vitro.Expression of BAX,BCL-2,Casepase-3 and Cyclin D1 protein were detected by Western blot.Results:The proportion of CD34^+CD38^--KG1 a stem cells was(95.35±2.63)%after immunomagnetic isolation.The proliferation of KGla stem cells was inhibited significantly by FA-2-b-β,which shows a time-and dose-dependent manner(24 h,r=0.943;48 h,r=0.976).Flow cytometry shows that with the increasing of drug concentration,the apoptosis was also increased,when KGla stem cells was treated by FA-2-b-βfor 24 h.Western blot indicated that the expression of apoptosis-related protein BAX and Casepase-3 were up-regulated,the expression of BCL-2 and Cyclin D1 were down-regulated.Conclusion:FA-2-b-βcan regulate proliferation and apoptosis KGla stem cells,the involved mechanism may be related with the activation of mitochondrial mediated apoptotic pathway.

关 键 词：秦巴硒菇 酸性RNA蛋白复合物 白血病干细胞 凋亡 线粒体 

分 类 号：R733.7[医药卫生—肿瘤]