泊马度胺的抗骨髓瘤细胞株MM1.S活性及对CRBN表达的影响  

Effect of Pomalidomide on Activity of Myeloma Cell Line MM1.S and Expression of CRBN

作　　者：樊文静[1] 范枝俏 潘耀柱[1] 杨柯[1] 尹娇娇[1] 赵宵晨 姚浩 白海[1] FAN Wen-Jing;FAN Zhi-Qiao;PAN Yao-Zhu;YANG Ke;YIN Jiao-Jiao;ZHAO Xiao-Chen;YAO Hao;BAI Hai(Department of Hematology,Center for Hematologic Diseases of Chinese PLA,Lanzhou Military Command General Hospital,Lanzhou 730050,Gansu Proince,China)

机构地区：[1]兰州军区兰州总医院全军血液病专科中心

出　　处：《中国实验血液学杂志》2019年第6期1907-1911,共5页Journal of Experimental Hematology

摘　　要：目的:探讨不同浓度泊马度胺对人多发性骨髓瘤细胞株MM1.S的作用及对CRBN表达的影响。方法:应用CCK-8法检测泊马度胺对MM1.S细胞增殖抑制作用;Annexin V-FITC/PI双染流式细胞术检测MM1.S细胞凋亡率;实时定量PCR检测CRBN基因表达水平;Western blot法检测泊马度胺对MM1.S细胞CRBN蛋白表达的影响。结果:泊马度胺对MM1.S细胞具有抑制作用,其增殖抑制作用呈时间和剂量依赖性(r=0.981,r=0.990);泊马度胺能够诱导MM1.S细胞凋亡;浓度为0、40和80μmol/L的泊马度胺作用于MM1.S细胞72 h后,CRBN基因表达水平分别为:1.487±0.340,0.211±0.054和0.055±0.005;泊马度胺作用后,CRBN蛋白表达水平明显下降,差异均有统计学意义。结论:泊马度胺能够抑制MM1.S细胞增殖并促进其凋亡,一定浓度泊马度胺可降低MM1.S细胞CRBN基因表达并下调其蛋白表达水平。Objective:To explore the effects of different concentration of pomalidomide on human multiple myeloma cell line MM1.S and the expression of CRBN.Methods:CCK-8 method was used for detecting inhibition effect of promalidomide on proliferation of MM1.S cells.Apoptosis rate of MM1.S cells was detected by flow cytometry with Annexin V-FITC/PI double staining.Real-time quantitative PCR was used to determine CRBN gene expression level.Western blot was used to detect the effect of pomalidomide on the protein expression of CRBN in MM1.S cells.Results:Pomalidomide has an inhibitory effect on MM1.S cells with time-and dose-dependent manners.Pomalidomide induced apoptosis in MM1.S cells.When the concentration of pomalidomide was 0,40 and 80μmol/L,the expression of CRBN gene after the treatment of MM1.S cells for 72 hours was 1.487±0.340,0.211±0.054 and 0.055±0.005,by using actin as internal refereme.Pomalidomide significantly reduced CRBN protein expression in MM1.S cells.Conclusion:Pomalidomide can inhibit the proliferation of MM1.S cells and promote its apoptosis.A certain concentration of pomalidomide can reduce the expression of CRBN gene and down-regulate its protein expression in MM1.S cells.

关 键 词：泊马度胺 Cereblon 骨髓瘤细胞株MM1.S 免疫调节剂 

分 类 号：R733.3[医药卫生—肿瘤]