脑心通胶囊对外周缺血组织炎症反应的作用及其机理  

Effect of Naoxintong Capsule on Inflammation in Peripheral Ischemic Tissue and Its Mechanism

作　　者：宋晓征 李成杰 伍雪英 SONG Xiao-Zheng;LI Cheng-Jie;WU Xue-Ying(Department of Neurology,Chengdu 363th Hospital,Chengdu 610041,Sichuan Province,China;Department of Radiological,Chengdu 363th Hospital,Chengdu 610041,Sichuan Province,China)

机构地区：[1]成都三六三医院神经内科,四川成都610041 [2]成都三六三医院放射科,四川成都610041

出　　处：《中国实验血液学杂志》2019年第6期1966-1972,共7页Journal of Experimental Hematology

摘　　要：目的:研究脑心通胶囊(nanxintong capsule,NXT)抑制外周组织缺血炎症的作用机理。方法:双盲法将实验小鼠随机均分为3组:假手术组、模型组和NXT组。模型组和NXT组动物接受后肢缺血手术(hind limb ischemia,HLI),并在术后1 h分别灌注生理盐水和NXT。术后3 d应用流式细胞术和免疫组织化学染色法检测腓肠肌中免疫细胞的比例;应用实时PCR和蛋白质芯片技术检测NXT诱发的基因和蛋白表达的变化,并对这些变化所涉及的生物过程进行分析。结果:与假手术组和模型组相比,NXT组腓肠肌炎症部位的噬中性粒细胞和巨噬细胞比例降低(P<0.001),基因EMR1的mRNA表达下调(P<0.001);炎症基因TNF-α、IL-1β的mRNA和蛋白表达降低(P<0.05)。蛋白质组学研究表明,NXT的使用改变了13种蛋白的表达模式,上调了6种细胞因子的表达量,降低了7种蛋白的分泌。此外,这13种蛋白质大多数参与了免疫细胞功能的调节。结论:NXT通过降低免疫细胞的比例及改变mRNA蛋白的表达模式抑制动脉缺血所引起的外周炎症反应,这为NXT临床上治疗外周炎症疾病提供了理论指导和潜在的靶位点。Objective:To study the mechanism of naoxintong capsule(NXT)-inhibiting peripheral ischemic inflammation.Methods:Mice were randomly double-blindly divided into 3 groups:sham-operation group,model group and NXT group.Both model and NXT groups underwent the hind limb ischemia(HLI)surgery followed by oral gavage with saline or NXT,respectively,one hour after operation.Three days after operation,the percentages of neutrophils and macrophages in the gastrocnemius muscle were examined by flow cytomety and immunohistochemical method.The changes in gene and protein expressions induced by NXT were examined by real-time PCR and protein chip,respectively.The changes of signaling pathways were analyzed.Results:Compared with sham oparation and model groups,NXT could decrease the ratios of neutrophils and macrophages in gastrocnemius inflammation site(P<0.01),and downregulate the mRNA expression of gene EMR1(P<0.01).NXT reduced the expression of TNF-αand IL-1βat both mRNA(P<0.001)and protein levels(P<0.05).The proteomic analysis showed that the use of NXT resulted in the expression changes of 13 proteins.The expression of 6 cytokines was increased,and the secretion of 7 proteins was upregulated.Besides,most of identified 13 proteins were involved in the function regulation of other immune cells.Conclusion:NXT can significantly alleviate ischemia-induced peripheral inflammation by reducing the ratio of immune cells and altering the expression patterns of mRNA and protein.The expression changes provide theoretical guidance and the potential targets for the clinical use of NXT in the treatment of ischemia-induced peripheral inflammatory diseases.

关 键 词：脑心通胶囊 缺血诱发的外周炎症 免疫细胞 细胞因子 mRNA/蛋白质表达模式 

分 类 号：R364.5[医药卫生—病理学] R273[医药卫生—基础医学]