基于指纹图谱和化学计量分析的鳕鱼肝油软胶囊掺假植物油鉴定  

Identification of adulterated vegetable oil in cod liver oil soft capsule based on fingerprint and chemometrics analysis

作　　者：王琼芬 刘婷 张梦奇 徐虹 石婧 WANG Qiong-Fen;LIU Ting;ZHANG Meng-Qi;XU Hong;SHI Jing({Zhoushan Institute for Food and Drug Control,Dinghai 316021,China)

机构地区：[1]舟山市食品药品检验检测研究院

出　　处：《食品安全质量检测学报》2019年第22期7787-7792,共6页Journal of Food Safety and Quality

基　　金：舟山市科技计划项目(2017C32079)~~

摘　　要：目的建立一种基于脂肪酸气相色谱(gas chromatography,GC)指纹图谱技术结合化学计量分析的鳕鱼肝油软胶囊中掺假植物油的鉴定方法。方法采用G C法测定不同来源鳕鱼肝油软胶囊脂肪酸指纹图谱,经拟合后构建对照指纹图谱,并进行样品相似度评价。模拟制备鳕鱼肝油软胶囊中掺人不同种类、不同比例植物油的掺假样品,以其中14个共有脂肪酸峰的相对峰面积作为数据源,输入S1MCA-P数据分析软件进行主成分分析(principal component analysis,PCA)及掺假模型建立。结果获得鳕鱼肝油软胶囊G C对照指纹图谱及三维掺假识别模型,44批样品经相似度评价发现有2批拟似掺假植物油,将拟似样品色谱数据标准化后导人SIMCA-P软件,显示2批样品均掺了大豆油,掺假比例约为15%和35%。结论脂肪酸G C指纹图谱结合化学计量分析为鳕鱼肝油软胶囊中掺假植物油的鉴定提供一种可靠准确的检测手段,可快速有效识别鳕鱼肝油掺假行为。Objective To establish a method for the identification of fatty acid-incorporated vegetable oil in codliver oil soft capsule based on fatty acid gas chromatography (GC) fingerprinting technique combined withchemometrics analysis. Methods The fatty acid fingerprints of cod liver oil soft capsules from different sourceswere determined by GC. The reference fingerprint was constructed after fitting and the similarity evaluation ofsamples were carried out. The adulterated samples of different types and different proportions of vegetable oil wereprepared by simulating the preparation of cod liver oil soft capsules. The relative peak areas of 14 common fatty acidpeaks were used as data sources. The SIMCA-P data analysis software was input for principal component analysis(PCA) and adulteration model establishment. Results The GC reference fingerprinting and the three-dimensionaladulteration identification model of the cod liver oil soft capsule was obtained. The similarity evaluation of 44batches of samples showed that there were 2 batches of samples suspected adulterated vegetable oil. After the sample chromatographic data were standardized and imported into SIMCA-P software, it was showed that the 2 batches ofsamples were adulterated with soybean oil, and the adulteration rate was about 1 5 % and 35%. Conclusion The fattyacid GC fingerprint combined with chemometrics analysis provides a reliable and accurate detection method for theidentification of adulterated vegetable oil in the cod liver oil soft capsule, which can quickly and effectively identifythe adulteration behavior of cod liver oil.

关 键 词：气相色谱法 指纹图谱 化学计量分析 脂肪酸 鳕鱼肝油软胶囊 掺假鉴定 

分 类 号：TS2[轻工技术与工程—食品科学与工程]