DNA错配修复与原发性肝癌易感性Meta分析  

Meta-analysis on DNA mismatch repair and primary hepatocellular carcinoma susceptibility

作　　者：韩亚男 秦盛 张晓琳[2] 刘晓刚[4] 贾金海[3] 刘英 HAN Ya-nan;QIN Sheng;ZHANG Xiao-lin;LIU Xiao-gang;JIA Jin-hai;LIU Ying(School of Public Health,North China University of Science and Technology,Tangshan 063000,P.R.China;Hebei Medical University,Shijiazhuang 050017,P.R.China;Department of Hepatobiliary Surgery,Tangshan Gongren Hospital,Tangshan 063000,P.R.China;Department of Science and Education,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,P.R.China)

机构地区：[1]华北理工大学公共卫生学院,河北唐山063000 [2]河北医科大学流行病与卫生统计学教研室,河北石家庄050017 [3]河北医科大学门诊部,河北石家庄050017 [4]唐山工人医院肝胆外科,河北唐山063000 [5]华北理工大学附属医院科教科,河北唐山063000

出　　处：《中华肿瘤防治杂志》2019年第21期1642-1647,共6页Chinese Journal of Cancer Prevention and Treatment

基　　金：河北省自然基金重大项目（H2016206576）

摘　　要：目的原发性肝癌是一种由遗传因素和环境因素共同作用而引起的多阶段、多因素复杂性疾病,是消化系统常见的恶性肿瘤之一。错配修复是遗传物质得以稳定遗传的保障,是DNA损伤修复的重要修复机制。本研究应用Meta分析的方法探讨错配修复蛋白与原发性肝癌易感性的关系。方法采用主题词和关键词"错配修复""错配修复蛋白""蛋白表达""免疫组化"和"肝癌",检索2018年以前在Pubmed、Ovid、EBSCO、Cochrane library、Science Direct、中国生物医学文献数据库、CNKI、万方数据库和维普数据库中发表的有关文献,收集关于DNA错配修复蛋白hMLH1表达异常与原发性肝癌易感性关系的病例对照研究,应用Review Manager 5.3和Stata 12.0进行数据合并分析。结果共纳入5篇相关文献,共计238例肝癌患者。文献经NOS量表评分均≥6,质量较高。异质性检验Q=9.02,P=0.06,I2=56%,存在异质性。采用随机效应模型,合并后OR=2.55(95%CI=1.09~5.97),Z=2.15,P=0.03。进一步以种族为亚组因素进行亚组分析显示,亚洲人群组无异质性,固定效应模型结果显示,OR=3.45(95%CI=1.89~6.28),Z=4.03,P<0.05。敏感性分析显示,合并后结果比较稳定。发表偏倚的Begg’s检验,Z=0.98,P=0.327;Egger’s检验,斜率为0.06,P=0.958,结果均显示不存在发表偏倚。结论 DNA错配修复hMLH1蛋白异常表达为原发性肝癌易感性的危险因素。OBJECTIVE Primary hepatocellular carcinoma is a multi-stage,multi-factor complex disease caused by genetic factors and environmental factors.It is one of the common malignant tumors in the digestive system.Mismatch repair is a guarantee for the stable inheritance of genetic material and an important repair mechanism for DNA damage.This study aimed to provide a quantitative summary in estimating the association between DNA mismatch repair protein and susceptibility to primary hepatocellular carcinoma(HCC)by Meta analysis.METHODS We searched databases(pubmed,Ovid,EBSCO,Cochrane library,ScienceDirect,SinoMed and CNKI)before 2018 by using "mismatch repair" or "Immunohistochemical",in combination with "primary liver cancer" and "primary hepatocellular carcinoma".A casecontrol study on the relationship between abnormal expression of DNA mismatch repair protein hMLH1 and susceptibility to primary hepatocellular carcinoma(HCC)was conducted.Data were analyzed by the software Review Manager 5.3 and Stata 12.0.RESULTS A total of 5 articles were included in this study,with the number of cases and controls of 238 and153,respectively.The scores of NOS were above 6,and the quality was high.Heterogeneity tests showed that the difference was statistically significant(Q=9.02,P=0.06,I2=56%).Therefore,using random effect model,the pooled OR=2.55(95%CI:1.09-5.97),significance test Z=2.15,P=0.03,the difference was statistically significant.Further subgroup analysis with race as subgroup factor showed that there was no heterogeneity in Asian population group,the pooled OR=3.45(95%CI:1.89-6.28),Z=4.03,P<0.05.Significance test had statistical significance.Sensitivity analysis showed that the combined results were relatively stable.Begg’s test of publication bias,Z=0.98,P=0.327.Egger’s test showed slope was 0.06,P =0.958,and the results showed that there was no publication bias.CONCLUSION Abnormal expression of hMLH1 protein by DNA mismatch repair can be considered as a risk factor for the susceptibility of primary hepatocellular carc

关 键 词：错配修复蛋白 HMLH1 原发性肝癌 免疫组织化学 META分析 

分 类 号：R73[医药卫生—肿瘤]