二氢杨梅素抑制人膀胱癌细胞增殖及其机制  

Dihydromyricetin inhibited the proliferation of human bladder cancer cells and its mechanism

作　　者：郭紫成 王子健 游文杰 陈洪波 肖宇 庄乾元[4] 王行环[1,2] Guo Zicheng;Wang Zijian;You Wenjie;Chen Hongbo;Xiao Yu;Zhuang Qianyuan;Wang Xinghuan(Department of Urologyy Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Human Genetics Resource Preservation Center in Hubei,Wuhan 430071,China;Department of Urology,The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China;Department of Urology,Tongji Hospital,Tongji Medical College of HUST,Wuhan 430030,China)

机构地区：[1]武汉大学中南医院泌尿外科,430071 [2]湖北省人类遗传资源保藏中心,武汉430071 [3]恩施土家族苗族自治州中心医院泌尿外科,445000 [4]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《中华实验外科杂志》2019年第12期2154-2156,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81772730)。

摘　　要：目的观察二氢杨梅素(DHM)对人膀胱癌细胞增殖的影响,并探讨其分子机制.方法用不同浓度(0、5、20μmol/L)DHM处理UMUC3细胞,通过噻唑蓝(MTT)实验和流式细胞术检测DHM对细胞增殖的抑制作用,实时荧光定量聚合酶链反应(FQ-PCR)和蛋白质印迹法(Westem blot)检测DHM对周期蛋白(Cyclin D1和Cyclin E1)以及周期蛋白依赖性激酶(CDK2和CDK4)的影响.用SPSS 21.0软件进行统计分析,结果采用均值±标准差(Mean±SD)表示,统计方法为单因素方差分析,多组间两两比较采用最小显著性差异(LSD)法和邓肯(Duncan)多重范围检验.结果DHM明显抑制UMUC3细胞增殖.与对照组[(48.4±0.6)%]比较,低浓度组[(55.2±0.1)%]和高浓度组[(60.5±0.3)%]G0/G1期细胞比例显著上升,差异有统计学意义(低浓度组F=417.668,P<0.01,高浓度组F=401.652,P<0.01).高浓度组G0/G1期细胞比例大于低浓度组,差异有统计学意义(F=100.221,P<0.05).RT-PCR和Western blot结果显示Cyclin D1、Cyclin E1、CDK2、CDK4表达均明显降低.结论DHM可抑制人膀胱癌细胞增殖,可能与阻滞细胞周期由G0/G1期向S期过渡有关.Objective To evaluate the effect and mechanism of dihydromyricetin(DHM)on the proliferation of human bladder cancer cells.Methods UMUC3 cells were incubated with DHM at the concentrations of 0,5 and 20 pimol/L respectively,then the inhibitory effect were investigated by thiazole blue(MTT)assay and flow cytometry.The expression of Cyclin DI and Cyclin El and cyclin-dependent kinases(CDK2 and CDK4)was detected using real-time fluorescence quantitativepolymerase chain reaction(FQ-PCR)and Western blotting.Statistical analysis was performed using SPSS 21.0 software and the results were expressed by mean value土standard deviation(Mean±SD).The statistical method was one-way analysis of variance incorporated with the least significant difference(LSD)method and Duncan multiple range test.Results DHM inhibited the proliferation of UMUC3 cells obviously.Compared with the control group[(48.4±0.6)%],the percentage of G0/Gx phase cells in low concentration group[(55.2±0.1)%]and high concentration group[(60.5±0.3)%]increased,and the difference was statistically significant(F=417.668 in the low concentration group,P<0.01,F=401.652 in the high concentration group,P<0.01).The percentage of Go/G]phase cells was higher in the high concentration group than in the low concentration group,and the difference was statistically significant(F=100.221,P<0.05).The results of RT-PCR and Western blotting showed that the expression of CDK2,CDK4,Cyclin DI and Cyclin El decreased obviously.Conclusion DHM can effectively inhibit the proliferation of human bladder cancer cells,and its mechanism may be related to blocking the transition of cell cycle from Go/G|phase to S phase.

关 键 词：二氢杨梅素 膀胱癌 增殖 细胞周期 

分 类 号：R73[医药卫生—肿瘤]