肝癌程序性死亡蛋白-1相关基因的生物信息学分析  

Bioinformatics analysis of programmed death protein-1 related genes in hepatocellular carcinoma

作　　者：任倩倩 朱鹏[2] 龚昭[2] Ren Qianqian;Zhu Peng;Gong Zhao(Department of Radiology,Union Hospital,Tongji Medical Collage,Huazhong University of Science and Technology,Hubei Province Key Laboratory of Molecular Imaging,Wuhan 430022,China;Department of Hepatobiliary Surgery,Wuhan No.1 Hospital,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院放射科分子影像湖北省重点实验室,武汉430022 [2]湖北省武汉市第一医院肝胆外科,武汉430022

出　　处：《中华实验外科杂志》2019年第12期2190-2192,共3页Chinese Journal of Experimental Surgery

基　　金：湖北陈孝平科技发展基金(CXPJJH11800001-2018203)。

摘　　要：目的筛选肝细胞癌中程序性死亡蛋白1(PD-1)相关基因及生物学通路.方法从癌症基因组图谱(TCGA)下载369例肝细胞癌患者转库组测序(RNA-seq)数据,对PD-1相关基因进行基因本体(GO)生物进程分析、京都基因与基因组百科全书(KEGG)信号通路分析,并构建蛋白与蛋白相互作用网络图(PPI),获得PD-1的关键靶基因,并对这些靶基因进行生存分析.结果共筛选PD-1相关基因174个.功能富集分析表明这些基因共涉及239个生物学过程,12种细胞成分和12个分子功能,这些基因主要参与T细胞活化及调节、免疫缺陷、T细胞增殖等.同时筛选出10个肝细胞癌PD-1相关基因的靶基因,其中ZAP70和FASLG的低表达与肝细胞癌的不良预后显著相关(0.137±0.118比0.377±0.083,0.204±0.156比0.284±0.106,χ^2=6.900、4.400,P<0.05).结论PD-1相关基因为肝细胞癌免疫治疗提供新突破点.Objective To screen the genes and biological pathways related to programmed death protein 1(PD-1)in hepatocellular carcinoma.Methods Download from the cancer genome atlas(TCGA)of 369 patients with hepatocellular carcinoma(HCC)transcriptome sequencing(RNA-seq)data,the PD-1 gene ontology(GO)to biological processes analysis,Kyoto encyclopedia(KEGG)signaling pathway gene and genome analysis,and construction of protein-protein interaction network diagram(PPI),key target genes for PD-1,and target genes for survival analysis.Results Altogether 174 PD-1 related genes were screened.Functional enrichment analysis showed that these genes involved 239 biological processes,12 cell components and 12 molecular functions.These genes were mainly involved in T cell activation and regulation,immune deficiency,T cell proliferation and so on.At the same time,10 key genes related to pd-1 in HCC were selected,and the low expression of ZAP70 and FASLG was significantly correlated with the poor prognosis of HCC(0.137±0.118 vs.0.377±0.083,0.204±0.156 vs.0.284±0.106,χ^2=6.900,4.400,P<0.05).Conclusion PD-1 gene provides a new breakthrough point for immunotherapy of hepatocellular carcinoma.

关 键 词：肝细胞癌 程序性死亡蛋白1 生物信息学 癌症基因组图谱 

分 类 号：R73[医药卫生—肿瘤]