榄香烯联合血管内皮生长因子多克隆抗体对大鼠脑胶质瘤脑组织增殖细胞核抗原、组织金属蛋白酶抑制剂-1表达的影响  

The effect of elemene in combination with vascular endothelial growth factor polyclonal antibody on the expression of proliferating cell nuclear antigen and tissue inhibitor of metalloproteinase-1 in brain glioma tissue of rats

作　　者：周江波[1] 王东[1] 马景鑑[2] 张建军[1] Zhou Jiangbo;Wang Dong;Ma Jingji;Zhang Jianjun(Department of Neurosurgery,Fourth Central Hospital of Tianjin,Tianjin 300140,China;Department of Neurosurgery,Tianjin No.1 Central Hospital,Tianjin 300192,China)

机构地区：[1]天津市第四中心医院神经外科,300140 [2]天津市第一中心医院神经外科,300192

出　　处：《中华实验外科杂志》2019年第12期2210-2213,共4页Chinese Journal of Experimental Surgery

基　　金：天津市自然科学基金(17JCYBJC273OO)。

摘　　要：目的观察榄香烯联合血管内皮生长因子(VEGF)多克隆抗体对大鼠脑胶质瘤脑组织增殖细胞核抗原(PCNA)、组织金属蛋白酶抑制剂-1(TIMP-1)表达的影响.方法采用脑内注射C6胶质瘤细胞建立大鼠胶质瘤模型,选取建模成功48只大鼠随机分为模型组、榄香烯组、VEGF多克隆抗体组、榄香烯联合VEGF多克隆抗体组,每组各12只.模型组腹腔注射0.5 ml生理盐水,榄香烯组腹腔注射0.5 ml榄香烯注射液,VEGF多克隆抗体组腹腔注射0.5 ml VEGF多克隆抗体,榄香烯联合VEGF多克隆抗体组腹腔注射0.5 ml榄香烯注射液、0.5ml VEGF多克隆抗体.隔日给药,连续7次.比较各组大鼠瘤体体积与抑瘤率、脑组织PCNA与TIMP-1表达.应用SPSS21.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,多组间比较采用单因素方差分析,两组间比较采用t检验.结果瘤体体积与抑瘤率:模型组、榄香烯组、VEGF多克隆组、榄香烯联合VEGF多克隆组大鼠瘤体体积分别为(68.54±8.12)、(37.65±6.24)、(39.12±6.41)、(30.12±5.24)mm3;香烯组、VEGF多克隆组、榄香烯联合VEGF多克隆组抑瘤率明显为(45.07±6.45)%、(42.92±6.52)%、(56.05±7.21)%.榄香烯组、VEGF多克隆组、榄香烯联合VEGF多克隆组大鼠瘤体体积明显小于对照组(t=10.449、9.851、13.772,P<0.01);榄香烯联合VEGF多克隆组大鼠瘤体体积明显小于榄香烯组、VEGF多克隆组(t=3.201、3.766,P<0.05),抑瘤率明显高于榄香烯组、VEGF多克隆组(t=3.932、4.679,P<0.05).脑组织PCNA与TIMP-1表达:模型组、榄香烯组、VEGF多克隆组、榄香烯联合VEGF多克隆组次瘤体组织PCNA指数分别为73.12±10.21、44.24±6.42、47.36±7.12、37.45±5.24;TIMP-1指数分别为45.24±6.45、62.45±8.15、59.45±7.32、78.65±11.24.榄香烯组、VEGF多克隆组、榄香烯联合VEGF多克隆组瘤体组织PCNA指数明显低于模型组(t=8.295、7.447、10.767,P<0.05),TIMP-1指数明显高于模型组(t=5.736、5.401、8.931,P<0.05);Objective To investigate the effect of elemene in combination with vascular endothelial growth factor(VEGF)polyclonal antibody on the expression of proliferating cell nuclear antigen(PCNA)and tissue inhibitor of metalloproteinase-1(TIMP-1)in brain glioma tissue of rats.Methods The rat glioma models were built by intracerebral injection of C6 glioma cells.Forty-eight successful rat models were selected and randomly divided into model group,elemene group,VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group,with 12 rats in each group.Rats in model group were performed intraperitoneal injection of 0.5 ml normal saline,rats in elemene group were performed intraperitoneal injection of 0.5 ml elemene injection,rats in VEGF polyclonal antibody group were performed intraperitoneal injection of 0.5 ml VEGF polyclonal antibody,and rats in elemene in combination with VEGF polyclonal antibody group were performed intraperitoneal injection of 0.5 ml elemene injection and 0.5 ml VEGF polyclonal antibody.The drug was administered on alternate days for 7 consecutive times.The tumour volume,tumor inhibition rate and the expression of PCNA and TIMP-1 in brain tissue of the rats between the groups were compared.Statistics with SPSS 21.0 software,measurement data for Mean±SD indicated that one factor variance analysis was used for the comparison between the two groups,and t test was used for the comparison between the two groups.Results(1)Tumor volume and tumor inhibition rate:The tumor volumes in model group,elemene group,polyclonal group,VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were(68.54±8.12),(37.65±6.24),(39.12±6.41),(30.12±5.24)mm',respectively;the tumor inhibition rates in elemene group,VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were(445.07±6.45)%,(42.92土6.52)%,(56.05±7.21)%,respectively.The tumor volume in elemene in combination with VEGF polyclonal antibody group was signif

关 键 词：脑胶质瘤 榄香烯 血管内皮生长因子多克隆抗体 增殖细胞核抗原 组织金属蛋白酶 抑制剂-1 

分 类 号：R73[医药卫生—肿瘤]