微小RNA-532-5p在胶质瘤组织的表达及其调控机制  

The expression of microRNA-532-5p in glioma and mechanism research

作　　者：黄绍崧[1] 林伟[1] Huang Shaosong;Lin Wei(Department of Neurosurgery,Fujian Provincial Hospital,Fuzhou 350001,China)

机构地区：[1]福建省立医院神经外科,福州350001

出　　处：《中华实验外科杂志》2019年第12期2276-2278,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测微小RNA(miRNA,miR)-532-5p在胶质瘤患者组织和正常脑组织中的表达水平,以及过表达miR-532-5p后对胶质瘤U251细胞增殖、迁移和侵袭能力的影响.方法收集30例胶质母细胞瘤患者的标本和30例外伤脑组织标本,使用实时定量反转录聚合酶链反应(RT-qPCR)法检测miR-532-5p的相对表达,分析其与患者临床病理因素之间的相关性.使用噻唑蓝(MTT)法、划痕实验和侵袭小室法(Transwell)观察转染miR-532-5p mimics后,对U251细胞的增殖、迁移和侵袭能力的影响.结果在胶质瘤组织与正常脑组织的比较中,胶质瘤组织miR-532-5p相对表达水平(0.381±0.310)明显低于正常脑组织(1.040±0.470,P<0.01).miR-532-5p表达水平与胶质瘤患者卡氏评分(KPS)和癫痫发生明显相关.胶质瘤细胞株U251的miR-532-5p相对表达量(0.280±0.080)明显低于脑星形胶质细胞株(NHA)(1.021±0.069,P<0.01).转染miR-532-5p后,U251细胞的增殖能力[(59.5±9.5)%]、迁移能力[(55.4±4.2)%,P<0.05]和侵袭能力[(45.7±6.3)%,P<0.01]明显下降.结论胶质瘤组织中的miR-532-5p表达水平呈低表达,且与胶质瘤患者KPS评分和癫痫发生明显相关.miR-532-5p与胶质瘤细胞的增殖、迁移和侵袭有关,过表达miR-532-5p能够明显抑制U251细胞的增殖、迁移和侵袭能力.Objective To explore the expression of microRNA(miRNA,miR)-532-5p in primary glioma and to detect the effects of miR-532-5p overexpression on proliferation,migration and invasion in U251 glioma cell line.Methods Real-time quantitative reverse transcriptase-polymerase chain reaction(RT-qPCR)was conducted to measure the expression of miR-532-5p in 30 cases of glioma tissues and traumatic brain specimens.We then analyzed the relationship of the level of miR-532-5p and the clinical pathological data of glioma patients.After transfection with miR-532-5p,the effects of on proliferation,migration and invasion were detected using methyl thiazol tetrazolium(MTT)assay,wound healing assay and transwell assay.Results The relative expression of miR-532-5p was significantly lower in glioma tissues(0.381±0.310)than in normal brain tissues(1.040±0.470,P<0.01).The level of miR-532-5p was significantly associated with Kamofsky(KPS)scores and seizures.Expression of miR-532-5p in U251 cell line(0.280±0.080)were significantly higher than those in Normal Human Astrocyte(NHA)cell line(1.021±0.069,P<0.01).The proliferation[(59.5±9.5)%,P<0.01],migration[(55.4±4.2)%,P < 0.05]an invasion[(45.7±6.3)%,P<0.01]of U251 cell with miR-532-5p mimic transfection were significantly lower than those in miR-532-5p negative control after transfection.Conclusion The expression of miR-532-5p were decreased in patients with glioma.The low level of miR-532-5p was significantly associated with the KPS scores and seizures.It was shown that miR-532-5p was associated with proliferation,migration and invasion in U251 cell line and high expression of miR-532-5p was significantly associated with the KPS scores and seizures Overexpression of miR-532-5p could inhibit the capability of proliferation,migration and invasion.

关 键 词：微小RNA-532-5p 胶质瘤 迁移 侵袭 病理因素 

分 类 号：R73[医药卫生—肿瘤]