过表达E3泛素连接酶CHIP对脑胶质瘤细胞U251周期及凋亡的影响  

Effect of the overexpression of E3 ubiquitin ligase carboxyl terminus of Hsc70-interacting protein on cell cycle and apoptosis in glioma cells U251

作　　者：陈洪福 张慧 梅鹏金 聂耳 CHEN Hongfu;ZHANG Hui;MEI Pengjin;NIE Er(Department of Neurosurgery,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,China)

机构地区：[1]徐州医科大学附属医院神经外科

出　　处：《中国实用神经疾病杂志》2019年第22期2437-2443,共7页Chinese Journal of Practical Nervous Diseases

基　　金：国家青年科学基金项目(编号:81802490)

摘　　要：目的观察E3泛素连接酶CHIP(Hsc70羧基末端相互作用蛋白)过表达质粒对脑胶质瘤细胞系U251周期及凋亡的影响。方法用pcDNA3.1空载质粒、pcDNA3.1-CHIP质粒转染脑胶质瘤细系U251,Western blot检测CHIP蛋白的表达,流式细胞仪检测CHIP蛋白过表达对U251细胞系周期及凋亡的影响。结果与对照组相比,CHIP组U251细胞CHIP蛋白表达量增加;与对照组相比,CHIP组U251细胞G0/G1期所占比例增加(P<0.01),S期(P<0.001)、G2/M期所占比例减少(P<0.05),与对照组相比,CHIP组U251细胞凋亡率增加(P<0.001)。结论 CHIP质粒的过表达可干扰CHIP蛋白的表达,CHIP作为肿瘤抑癌因子抑制胶质瘤细胞U251 G0/G1期进展,促进胶质瘤细胞U251凋亡。Objective To observe the effect of the overexpression of CHIP plasmid on cell cycle and apoptosis in glioma cells.Methods We use pcDNA3.1 light plasmid and pcDNA3.1-CHIP plasmid respectively to transfect glioma cells U251 cells.The expression levels of CHIP in glioma cells U251 were detected after CHIP overexpression by western blot.The influence on the cell cycle and apoptosis of glioma cells U251 after CHIP overexpression were measured by flow cytometry.Results To compare Control plasmid group with CHIP overexpression plasmid group the latter could increase the expression of CHIP in glioma cells U251.To compare Control plasmid group with CHIP overexpression plasmid group the latter could increase G0/G1 phase ratio of cell cycle(P<0.01) and decrease the S(P<0.001),G2/M phase ratio of cell cycle in glioma cells U251(P<0.05).To compare Control plasmid group with CHIP overexpression plasmid group the latter could contribute to the rate of apoptosis increased in glioma cells U251(P<0.001).Conclusion CHIP overexpression targeted interference the expression levels of CHIP,CHIP acts as a tumor suppressor that inhibit the G0/G1 phase of glioma cells U251 progress and contribute to glioma cells U251 apoptosis.

关 键 词：热休克蛋白70羧基末端相互作用蛋白(CHIP) E3泛素连接酶 胶质瘤细胞 细胞周期 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]