抗酒石酸酸性磷酸酶5在结直肠癌中的表达及临床意义  

Clinical significance of expression of tartrate-resistant acid phosphatase 5 in colorectal cancer

作　　者：虞佳音 王振 李鑫 杨宏杰 张琳 汪静宇 YU Jiayin;WANG Zhen;LI Xin;YANG Hongjie;ZHANG Lin;WANG Jingyu(Department of Pathology,The First Hospital of Jiaxing City in Zhejiang Province,Jiaxing 314000,China)

机构地区：[1]浙江省嘉兴市第一医院病理科

出　　处：《中国现代医生》2019年第33期131-135,I0002,共6页China Modern Doctor

基　　金：浙江省自然科学基金(LY15H160067);浙江省嘉兴市科技计划项目(2016AY23043);浙江省嘉兴市第一医院院级课题(2017QMX001)

摘　　要：目的研究抗酒石酸酸性磷酸酶5(ACP5)在结直肠癌(CRC)中的表达,并探讨其临床意义。方法采用酶联免疫吸附试验(ELISA)检测2017年1月~2018年4月35例结直肠癌患者和46例正常健康人血清中ACP5的水平;采用免疫组织化学(IHC)技术检测160例CRC组织中ACP5蛋白的表达情况;采用统计学方法对ACP5蛋白水平及临床病理特征进行评估。结果与正常健康对照组相比,结直肠癌患者血清ACP5水平明显升高(P=0.0071);IHC结果显示,淋巴结转移组伴随ACP5高表达(P=0.003);统计分析结果显示,ACP5高表达的患者生存期较短(P=0.001),ACP5表达与淋巴结转移相关。结论 ACP5有可能成为预测CRC淋巴转移的标志物,其在CRC组织中的表达是影响预后的不良因素。Objective To investigate the expression of tartrate-resistant acid phosphatase 5(ACP5)in colorectal cancer(CRC)and to explore its clinical significance.Methods The enzyme-linked immunosorbent assay(ELISA)was used to detect the serum ACP5 levels of 35 patients with colorectal cancer and 46 normal healthy individuals from January2017 to April 2018.The immunohistochemical method(IHC)was used to detect the expression of ACP5 protein in 160 cases of CRC tissues.Statistical methods were used to evaluate the ACP5 protein levels and clinicopathological features.Results It was found by ELISA that the serum ACP5 levels in the patients with colorectal cancer were significantly elevated compared to those in the normal healthy controls(P=0.0071).It was found by IHC that the lymph node metastasis group was associated with high expression of ACP5(P=0.003).It was found by statistical analysis that the survival time was shorter for the patients with higher expression of ACP5(P=0.001)and that ACP5 expression was associated with lymph node metastasis.Conclusion ACP5 may serve as a marker for predicting CRC lymphatic metastasis and that its expression in CRC tissue is an adverse factor affecting prognosis.

关 键 词：ACP5 结直肠癌 转移标志物 免疫组化 酶联免疫吸附试验 

分 类 号：R735.3[医药卫生—肿瘤]