Transcriptome analysis of carotenoid biosynthesis in Dunaliella salina under red and blue light  

作　　者：LI Yuanxiang CAI Xuehua GU Wenhui WANG Guangce 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China [3]College of Earth Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2020年第1期177-185,I0016-I0018,共12页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.41506188);the China Nantong Municipal Applied Basic Research Program(No.MS12017025-2);the Tianjin Demonstration Project for Innovative Development of Marine Economy(No.BHSF2017-21)

摘　　要：The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms.In this study,we exposed the unicellular green alga Dunaliella salina to red(660 nm)and blue(450 nm)light and analyzed the cell growth,total carotenoid content,and transcriptomes.The growth of D.salina was enhanced by illumination with red light,whereas blue light was not able to promote the algal growth.In contrast,the total carotenoid content increased under both red and blue light.The RNA of D.salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated.Six transcripts encoding for the blue light receptor cryptochrome were identified,and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light.Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light.The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D.salina under different wavelengths of light.The quality of light is an important abiotic factor that af fects the growth and development of photosynthetic organisms. In this study, we exposed the unicellular green alga Dunaliella salina to red(660 nm) and blue(450 nm) light and analyzed the cell growth, total carotenoid content, and transcriptomes. The growth of D. salina was enhanced by illumination with red light, whereas blue light was not able to promote the algal growth. In contrast, the total carotenoid content increased under both red and blue light. The RNA of D. salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated. Six transcripts encoding for the blue light receptor cryptochrome were identi?ed, and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light. Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light. The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D. salina under dif ferent wavelengths of light.

关 键 词：blue LIGHT CAROTENOID DUNALIELLA RED LIGHT TRANSCRIPTOME 

分 类 号：R73[医药卫生—肿瘤]