长链非编码RNA CTD-3162L10.1调节SEMA3B表达对卵巢癌细胞增殖和侵袭的影响  

Effect of long-chain non-coding RNA CTD-3162L10.1 on the proliferation and invasion of ovarian cancer cells by regulating the expression of SEMA3B

作　　者：魏峰[1] 张锦[1] 张逸群[1] 郑蓉[1] WEI Feng;ZHANG Jin;ZHANG Yiqun;ZHENG Rong(Department of Gynecology,Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]湖北医药学院附属太和医院妇科

出　　处：《国际检验医学杂志》2019年第24期2982-2985,2990,共5页International Journal of Laboratory Medicine

摘　　要：目的分析卵巢癌中长链非编码RNA CTD-3162L10.1对信号素3B(SEMA3B)表达的调节作用及对卵巢癌细胞增殖和侵袭的影响。方法实时定量聚合酶链式反应(qPCR)检测23对卵巢癌和癌旁组织中CTD-3162L10.1的表达。qPCR检测卵巢癌细胞株A2780、SKOV-3、HO-8910、OC3、OVCAR-3和人健康卵巢上皮细胞IOSE80中CTD-3162L10.1的表达。以CTD-3162L10.1表达水平最低的卵巢癌细胞株为转染对象,分别转染空载质粒和表达CTD-3162L10.1的质粒,命名为对照组和实验组。qPCR检测转染效率。四甲基偶氮唑蓝(MTT)法和Transwell侵袭实验分别检测高表达CTD-3162L10.1对卵巢癌细胞增殖和侵袭能力的影响。qPCR检测SEMA3B mRNA的表达,蛋白免疫印迹法(Western blot)检测SEMA3B、胰岛素样生长因子结合蛋白-6(IGFBP-6)、磷酸化的蛋白激酶B(p-AKT)蛋白的表达。结果CTD-3162L10.1在卵巢癌和癌旁组织中的表达量分别为(1.05±0.35)和(7.28±1.67),卵巢癌组织中CTD-3162L10.1呈低表达(P<0.05)。与人健康卵巢上皮细胞相比,卵巢癌细胞株中CTD-3162L10.1呈低表达(P<0.05),OVCAR-3细胞中的表达量最少(P<0.05)。与对照组相比,实验组OVCAR-3细胞中CTD-3162L10.1表达明显增加(P<0.05),表明转染成功。与对照组相比,CTD-3162L10.1可明显抑制实验组OVCAR-3细胞的增殖能力和侵袭能力(P<0.05)。与对照组相比,实验组OVCAR-3细胞中SEMA3B mRNA表达明显增加(P<0.05)。SEMA3B、IGFBP-6蛋白表达增加,p-AKT蛋白表达降低。结论CTD-3162L10.1在卵巢癌中呈低表达,高表达CTD-3162L 10.1可抑制卵巢癌细胞OVCAR-3的增殖能力和侵袭能力,其分子机制可能是通过促进SEMA3B基因表达实现。Objective To analyze the regulation of long-chain non-coding RNA CTD-3162L10.1 in ovarian cancer on the expression of semaphorin 3B(SEMA3B)and its effect on proliferation and invasion of ovarian cancer cells.Methods qPCR was used to detect the expression of CTD-3162L10.1 in ovarian cancer and adjacent tissues.qPCR was used to detect the expression of CTD-3162L10.1 in ovarian cancer cell lines A2780,SKOV-3,HO-8910,OC3,OVCAR-3 and human normal ovarian epithelial cells IOSE80.The ovarian cancer cell line with the lowest expression level of CTD-3162L10.1 was transfected and transfected with empty plasmid and plasmid expressing CTD-3162L10.1,named as control group and experimental group.qPCR was used to detect transfection efficiency.Methyl thiazolyl tetrazolium(MTT)assay and Transwell invasion assay were used to detect the effect of high expression of CTD-3162L10.1 on the proliferation and invasion of ovarian cancer cells.The expression of SEMA3B mRNA was detected by qPCR.The expressions of SEMA3B,E-cadherin,N-cadherin,zinc finger transcription factor Snail and zinc finger transcription factor Slug protein were detected by Western blot.Results The expression levels of CTD-3162L10.1 in ovarian cancer and adjacent tissues were(1.05±0.35)and(7.28±1.67)respectively.CTD-3162L10.1 was lowly expressed in ovarian cancer tissues(P<0.05).Compared with normal ovarian epithelial cells,CTD-3162L10.1 was down-regulated in ovarian cancer cell lines(P<0.05),and OVCAR-3 cells were least expressed(P<0.05).Compared with the control group,the expression of CTD-3162L10.1 in the OVCAR-3 cells of the experimental group was significantly increased(P<0.05),indicating successful transfection.Compared with the control group,CTD-3162L10.1 significantly inhibited the proliferation ability and invasion ability of the OVCAR-3 cells in the experimental group(P<0.05).Compared with the control group,the expression of SEMA3B mRNA in the OVCAR-3 cells of the experimental group was significantly increased(P<0.05).The expression of SEMA3B and IGFBP-6 p

关 键 词：CTD-3162L10.1 卵巢癌 细胞增殖 细胞侵袭 

分 类 号：R737.31[医药卫生—肿瘤]