内源性VEGF对TMP促进大鼠脊髓损伤修复的影响  

The Effect of Endogenous VEGF on the Recovery of Spinal Cord Injury in Rats Treated with TMP

作　　者：杜宝玲[1] 刘玉荣[1] 高志岩[2] 张征宇[3] DU Baoling;LIU Yurong;GAO Zhiyan;ZHANG Zhengyu(Department of Histology&Embryology,Guangzhou Medical University,Guangzhou 511436,China;Morphology Experimental Center,Guangzhou Medical University,Guangzhou 511436,China;Guangzhou Institute of Cardiovascular Disease,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China)

机构地区：[1]广州医科大学组织学与胚胎学教研室,广东广州511436 [2]广州医科大学形态学实验中心,广东广州511436 [3]广州医科大学附属第二医院心血管研究所,广东广州510260

出　　处：《临床医学工程》2019年第12期1651-1652,共2页Clinical Medicine & Engineering

基　　金：广东省中医药局面上科研项目（项目编号：20171181）

摘　　要：目的观察VEGF对TMP治疗大鼠脊髓损伤的影响。方法实验大鼠随机分为假手术组、生理盐水组、TMP组和TMP+Avastin组,观察术后8周脊髓损伤处VEGF、vWF和IL-8表达,以及神经纤维再生、后肢运动功能。结果 TMP+Avastin组内源性VEGF表达减少,限制血管新生及炎性反应(P<0.05)。对于神经纤维再生及其后肢运动,TMP组优于生理盐水组(P<0.05), TMP+Avastin组的效果更优(P<0.05)。结论内源性VEGF阻碍了TMP对大鼠脊髓损伤的修复,Avastin通过降低炎性反应提高TMP治疗大鼠脊髓损伤的疗效,促进神经纤维再生及后肢运动功能恢复。Objective To observe the effect of endogenous VEGF on the recovery of spinal cord injury in rats treated with TMP.Methods The rats were randomly divided into Sham group, NS group, TMP group and TMP + Avastin group. 8 weeks after operation, the expressions of VEGF, v WF and IL-8, the regeneration of nerve fibers in the injury area, and the motor function of hind limbs were observed.Results Avastin effectively suppressed VEGF expression and led to a limitation inangiogenesis and inflammatory response(P<0.05). The nerve fibers regeneration and BBB scores: TMP + Avastin group > TMP group > NS group(P<0.05). Conclusions Endogenous VEGF limits the effect of TMP on the recovery of spinal cord injury in rats. Avastin improves the effect of TMP on the recovery of spinal cord injury through reducing angiogenesis and inflammation.

关 键 词：TMP VEGF AVASTIN 脊髓损伤 

分 类 号：R744[医药卫生—神经病学与精神病学]