琼玉膏对胰腺癌小鼠化疗增效减毒作用研究  

Synergistic and attenuating effects of Qiongyugao on pancreatic cancer mice with chemotherapy

作　　者：刘丽[1] 刘大晟[2] 李震东[3] 李接兴[3] 吴先林[4,5] LIU Li;LIU Da-sheng;LI Zhen-dong;LI Jie-xing;WU Xian-lin(Department of Outpatient,The First Affiliated Hospital of Jinan University,Guangzhou 510632,China;Department of Vascular and Thyroid Surgery,Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120,China;Department of Hepatobiliary Surgery,Guangzhou 510632,China;Center for Diagnosis and Treatment of Pancreatic Diseases,Guangzhou 510632,China;Central Laboratory,The First Affiliated Hospital of Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学附属第一医院校门诊部,广东广州510632 [2]广东省中医院血管甲状腺科,广东广州510120 [3]暨南大学附属第一医院肝胆外科,广东广州510632 [4]暨南大学附属第一医院中西医结合胰腺病诊治中心,广东广州510632 [5]暨南大学附属第一医院中心实验室,广东广州510632

出　　处：《中国病理生理杂志》2019年第12期2181-2186,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81403302);广东省中医药局科研基金资助项目(No.20182021);中央高校基本科研业务费专项资金资助项目(No.21617499);暨南大学第一临床医学院科研培育专项基金资助项目(No.2017212)

摘　　要：目的:观察琼玉膏对胰腺癌小鼠吉西他滨化疗的增效减毒作用并探讨其可能机制。方法:50只C57BL/6小鼠随机均分为5组:正常组、模型组、吉西他滨治疗组、琼玉膏治疗组和联合治疗(吉西他滨+琼玉膏)组,模型组和治疗组均采用胰腺癌Panc02细胞株皮下接种建立胰腺癌小鼠移植瘤模型,分别给予生理盐水、吉西他滨、琼玉膏以及吉西他滨联合琼玉膏治疗。观察各组小鼠瘤体体积、血常规和肿瘤标志物的变化,采用流式细胞术检测各组小鼠外周血中T细胞亚群Th1/Th2和Th17/Treg的表达。结果:Panc02细胞皮下接种可成功建立胰腺癌小鼠移植瘤模型。与模型组比较,琼玉膏、吉西他滨及联合治疗均可减缓瘤体增长(P<0.05),降低糖链抗原19-9(CA19-9)和癌胚抗原(CEA)浓度(P<0.05)。琼玉膏还可降低Th1/Th2和Th17/Treg比例(P<0.05),并增加吉西他滨化疗小鼠白细胞(WBC)、红细胞(RBC)和血小板(PLT)计数及血红蛋白(HGB)含量。结论:琼玉膏可能通过调节T细胞亚群平衡对胰腺癌起到一定的治疗作用,并对吉西他滨化疗产生一定的增效减毒作用。AIM:To observe the synergistic and attenuating effects of Qiongyugao on pancreatic cancer mice with chemotherapy and to explore its possible mechanism.METHODS:C57BL/6 mice(n=50)were randomly divided into normal group,model group,gemcitabine treatment group,Qiongyugao treatment group,and combined treatment(gemcitabine+Qiongyugao)group.The pancreatic cancer mouse xenograft model was established by subcutaneous inoculation with pancreatic cancer Panc02 cells among the mice in model group and 3 treatment groups.Normal saline,gemcitabine,Qiongyugao and gemcitabine plus Qiongyugao were also used accordingly.The tumor growth curve,blood routine and tumor markers were observed in each group.Furthermore,flow cytometry was used to detect the expression of Th1/Th2 and Th/Treg in peripheral blood of the mice.RESULTS:Subcutaneous vaccination with Panc02 cells successfully established a transplanted tumor model of pancreatic cancer.Compared with model group,Qiongyugao,gemcitabine and the combination of the 2 drugs inhibited tumor growth(P<0.05),and decreased the concentration of carbohydrate antigen 19-9(CA19-9)and carcinoembryonic antigen(CEA)(P<0.05).Qiongyugao decreased the ratio of Th1/Th2 and Th17/Treg,and increased the white blood cell(WBC),red blood cell(RBC)and platelet(PLT)counts and hemoglobin(HGB)content in gemcitabine treated mice(P<0.01).CONCLUSION:Qiongyugao may have a certain therapeutic effect on pancreatic cancer and produce certain synergistic and attenuating effects on gemcitabine chemotherapy.

关 键 词：琼玉膏 胰腺癌 Th/Treg平衡 吉西他滨 

分 类 号：R730.23[医药卫生—肿瘤] R735.9[医药卫生—临床医学]