IL-1和IL-6诱导小鼠红细胞膜磷脂酰丝氨酸外露及其相关衰亡特征的研究  

IL-1 and IL-6 induced phosphatidylserine exposure on erythrocyte membrane and related characteristics of eryptosis in mice

作　　者：王延媛 沈华 马爽 南勇 刘鸿翔 WANG Yanyuan;SHEN Hua;MA Shuang;NAN Yong;LIU Hongxiang(Emergency Critical Care Division,Central Hospital of Songjiang District of Shanghai,Songjiang Branch of First People’s Hospital Affiliated to Shanghai Jiaotong University,Shanghai,201699,China)

机构地区：[1]上海市松江区中心医院(上海交通大学附属第一人民医院松江分院)急诊危重病科

出　　处：《临床急诊杂志》2019年第11期889-894,共6页Journal of Clinical Emergency

基　　金：上海市松江区医药卫生医学领先专业项目(No:2012-Ⅲ-05)

摘　　要：目的:探讨IL-1和IL-6在诱导小鼠红细胞衰亡特征性表现中的作用。方法:将分离的小鼠红细胞经IL-1和IL-6分别处理6、12、24、48 h或在不同浓度(0.1、1.0、10.0 ng/ml)处理24 h后,利用流式细胞仪检测红细胞前向散射值(FSC)的变化,红细胞膜磷脂酰丝氨酸(PS)和神经酰胺的标记率。结果:经IL-1或IL-6处理后的小鼠红细胞相较对照组于24 h后出现FSC减小,分别为[(81.9±1.86)%、(82.13±2.18)%vs.(87.6±0.55)%,P<0.05],膜的PS外露水平和神经酰胺含量增加,分别为[(5.7±0.62)%、(5.73±0.45)%vs.(2.7±0.17)%,P<0.01]和[(2.4±0.15)%、(2.0±0.36)%vs.(0.7±0.26)%,P<0.01],并随着时间的推移变化趋势更加明显。结论:IL-1和IL-6均可诱导红细胞体积缩小,促进红细胞膜PS外露以及神经酰胺的增加,是小鼠红细胞衰亡的触发因素。Objective:To investigate the role of IL-1 and IL-6 on characteristics of eryptosis in mice.Method:Erythrocytes isolated from mice were treated by IL-1 or IL-6 at the dose of 1 ng/ml for 6,12,24 and 48 hours or at different concentrations(0.1,1,10 ng/ml)for 24 hours.The forward scatter(FSC),phosphatidylserine(PS)exposure and ceramide formation were determined by flow cytometry.Result:Compared to control group,the decrease of FSC[(81.9±1.86)%,(82.13±2.18)%vs.(87.6±0.55)%,P<0.05]and the increase of membrane PS exposure level and ceramide content[(5.7±0.62)%,(5.73±0.45)%vs.(2.7±0.17)%,P<0.01],[(2.4±0.15)%,(2.0±0.36)%vs.(0.7±0.26)%,P<0.01]were observed in erythrocyte treated by IL-1 or IL-6 for 24 h respectively.More obvious tendency were followed with the passage of time.Conclusion:IL-1 or IL-6 can trigger eryptosis by inducing cell shrinkage,more PS exposure and ceramide formation on the membrane of erythrocyte in mice.

关 键 词：白介素1 白介素6 红细胞衰亡 

分 类 号：R363[医药卫生—病理学]