绿原酸的提纯分析技术及策略  

Technology and Strategy for Extraction and Purification of Chlorogenic Acid

作　　者：徐尤美 蔺蓓蓓 郑红星 陈琛 XU You-mei;LIN Bei-bei;ZHENG Hong-xing;CHEN Chen(Chinese-German Joint Institute for Natural Product Research,College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China)

机构地区：[1]陕西理工大学生物科学与工程学院中德天然产物研究所

出　　处：《化学试剂》2019年第12期1260-1270,共11页Chemical Reagents

基　　金：国家高端外国专家项目(GDT20186100426);国家重点引智项目(W20166100045);陕西省科技研究发展计划项目(2017NY-082);陕西省教育厅科学研究计划(17JK0137);陕南秦巴山区生物资源综合开发协同创新中心资助项目(QBXT-Z(P)-15-25)

摘　　要：绿原酸具有抗菌、抗病毒、抗炎、保肝、降糖降脂、调节免疫、神经保护、降压、抗氧化等多种生物活性,临床上可用于抗菌解毒、消炎利胆。绿原酸具有易氧化、稳定性差,同分异构体较多且性质差异小,提取纯化难度大,导致绿原酸的提取率低,成本高,因此绿原酸的高效提制是天然产物研究中一个热点之一。重点概述了绿原酸的提取方法,包括传统提取法、物理提取法、酶解法、超临界流体萃取法。绿原酸的纯化方法,包括膜分离、聚酰胺柱层析、大孔吸附树脂分离、高效液相色谱、高速逆流色谱法,并分析了各种方法的优缺点和应用效果,提出了绿原酸提取纯化的技术方案,为建立高效低廉的高纯度绿原酸提取纯化提供参考。The chlorogenic acid possess extensive physiological activity of antivirus,anti-inflammatory,hepatic-protective,hypoglycemic and hypolipidemic,immune regulation,neuroprotective,decreasing blood pressure,anti-oxidation and so on.It can be used for antibacterial disinfection,anti-inflammatory and choleretic in clinically.Due to the unique physical and chemical properties of chlorogenic acid(easy oxidation,poor stability,more isomers and small difference in properties),it is difficult to extract and purify,resulting in low extraction rate and high cost of chlorogenic acid.Therefore,the efficient extraction of chlorogenic acid is one of popular topics natural product.However,very few works have focused on reviewing the different techniques for extraction of chlorogenic acid.Extraction methods of chlorogenic acid,focuses on traditional extraction,the physical extraction,enzyme hydrolysis,and supercritical fluid extraction were reviewed.The purification method of chlorogenic acid including membrane separation,column chromatography on polyamide,macroporous adsorption resins,high performance liquid chromatography,and high-speed countercurrent chromatography.The advantages and disadvantages of the extraction and purification techniques of chlorogenic acid were analyzed.The technical scheme of extraction and purification of chlorogenic acid was proposed,which provided a reference for the establishment of high-efficiency and low-cost extraction and purification of high purity chlorogenic acid.

关 键 词：绿原酸 提取 纯化 生物活性 高纯度 

分 类 号：TS201[轻工技术与工程—食品科学]