B-连环蛋白在兔下颌骨牵引区新生骨组织中的表达  

The expression of p-catenin in callus of distraction gap during mandibular distraction osteogenesisin rabbits

作　　者：戴浩[1] 张春丽[1] 上官文松 胡纯兵[1] 王树 吴国平[1] Dai Hao;Zhang Chunli;Shangguan Wensong;Hu Chunbing;Wang Shu;Wu Guoping(Department of Plastic Surgery,the Affiliated Friendship Plastic Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学附属友谊整形外科医院整形外科,210029

出　　处：《中华整形外科杂志》2019年第11期1128-1134,共7页Chinese Journal of Plastic Surgery

基　　金：江苏省自然科学基金(BK20151075)。

摘　　要：目的检测连环蛋白(P-catenin)在兔下颌骨牵引区新生骨组织中的表达,探讨p-catenin在牵引成骨新骨形成中的作用。方法将26只新西兰大白兔采用随机数字表法分为3组:正常对照组(A组)2只、下颌骨缺损对照组(B组)12只、下颌骨牵引组(C组)12只。A组2只兔不行手术,其下颌骨作为正常对照;B组于第1磨牙和额孔间作下颌骨垂直截骨后随即牵开5 mm,用钛板、钛钉坚强内固定;C组作相同截骨后,断端复位,放置牵引器固定,术后第4天开始以0.8 mm/d的速度连续牵引7 d,然后进入固定期。B、C组分别于第6、10、17、24、31、38天处死2只兔,切取牵引区新生骨痂行蛋白质印迹法和免疫组织化学方法检测p-catenin的分布及表达情况,并做图像分析。测量结果通过SPSS 22.0统计软件利用斯皮尔曼等级相关系数进行数据分析,r>0.75,P<0.05为差异有统计学意义。结果蛋白质印迹法检测结果显示,牵引期(术后6~10 d)P-catenin的表达水平增高,在牵引末期(术后10 d)达到顶峰,进入固定期后p-catenin的表达水平逐渐下降。各时期C组3-catenin的表达水平较B组高。免疫组织化学染色显示,p-catenin主要在骨痂组织的炎细胞(如单核细胞)、成纤维细胞及少量沿牵张方向排列的新生成骨细胞、骨细胞和骨周围结缔组织中表达,胞浆和胞核染色呈阳性。C组在牵引开始后(术后第6天)》catenin的表达强烈(3.2458±0.1323),在第10天达高峰(4.6028±0.0219)o随着牵引张力的消失,术后第17天开始,P-catenin表达逐渐降低(3.6398±0.1255),但染色仍为阳性。B组在术后第6天p-catenin染色强阳性(2.7340±0.1347),术后第10天最为强烈(3.1013±0.1048),术后第17天表达为2.5428±0.2111,较术后第10天减弱。各时间点牵引组p-catenin表达高于下颌骨缺损对照组,差异有统计学意义(r=0.943,P=0.0056)o结论在下颌骨牵引成骨过程中,牵张应力使p-catenin表达增强,提示P-catenin在从机械信号�Objective To explore the role of p-catenin in distraction osteogenesis of new boneformation, the expression of p-catenin in the distraction gap callus was detected during rabbit mandibulardistraction osteogenesis. Methods 26 New-Zealand rabbits were randomly divided into 3 groups. Group Ais a normal control group with 2 rabbits, group B is the mandibular defect control group, and group C is thedistraction group. Group B and C with 12 rabbits, respectively. The two rabbits in group A without surgery,their mandibles are normal control. In group B, vertical osteotomy was performed between the first molarand the mental foramen on the mandibles bilaterally, followed by rigid internal fixation with titanium plates and screw with 5 mm gap immediately. In group C , after the same osteotomy was performed , the fragmentsof mandibles were reduced and fixed with mandibular distractors bilaterally. On the fourth daypostoperatively, the distraction started at a rate of 0. 8 mm/d and lasted for 7 days, followed byconsolidation period. Two rabbits of group B and C were sacrificed at 6th, 10th, 17th , 24th , 31st, 38th daypostoperatively, respectively. The newly formed callus in the distraction gap of mandibles was harvested forWestern blotting and immunohistochemistry examination to detect the distribution and expression of pcatenin.The experimental data were analyzed by SPSS 22. 0 statistical software using Spearman function.Results The result of Western blotting showed that the expression of p-catenin gradually increased atdistraction period ( 6-10 days after surgery) and reached the peak at the end of the distraction period( 10thday postoperatively) , it gradually decreased during the consolidation period. However, the expression of (3-catenin in group C was higher than that of group B. Immunohistochemistry stain showed that the expressionof |3-catenin mainly located in inflammatory cells ( eg. monocyte) , fibroblast of the granulation tissue, theosteoblasts, osteocyte on the surface of new formed trabecular, and the connecti

关 键 词：连环蛋白 下颌骨 骨生成 牵张 蛋白质印迹法 免疫组织化学 

分 类 号：R78[医药卫生—口腔医学]