甲烷氧化菌与光合菌协同培养高产单细胞蛋白  

The production of single cell protein by methanotrophs and photosynthetic bacteria co-culture

作　　者：孙立瑞 辛嘉英[1,2] 邓晓萍 SUN Li-rui;XIN Jia-ying;DENG Xiao-ping

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076 [2]中国科学院兰州物理化学研究所,甘肃兰州730000

出　　处：《饲料研究》2019年第11期64-69,共6页Feed Research

基　　金：国家自然科学基金项目(项目编号：21573055);哈尔滨商业大学创新团队项目(项目编号：YJSCX2018-481HSD);山西省人才专项(优秀人才科技创新)项目(项目编号：201705D211029)

摘　　要：单细胞蛋白(SCP)是在一定条件下培养微生物个体而获取的微生物蛋白,广泛应用于畜牧养殖业中。为提高SCP产量开发了1种以甲烷氧化菌和光合菌为混合菌种,采用共培养技术高产SCP的方法。研究确定了最佳生长条件,当气体比例CH4:O-2:CO2为2:2:1、换气时间间隔24 h、光照强度20μmol/(m^2·s)时,细胞的延滞期(λ)最短,为15.37 h;最大比生长速率(μmax)最高,为0.0031 h^-1,产SCP干重为0.173 g/100 mL,试验讨论了几种金属离子(Cu^2+、Mg^2+、Fe^3+)对共培养体系细胞生长情况的影响。研究表明加入光合菌的甲烷氧化菌共培养体系能够明显促进甲烷氧化菌生长、缩短发酵周期、显著提高SCP产量。Single Cell Protein(SCP)is a microbial protein obtained by cultivating microbial individuals under certain conditions and is commonly used in animal husbandry and aquaculture.In order to increase the production of SCP,a method of producing SCP with co-culture technology using a mixture of methanotrophs and photosynthetic bacteria was developed.The optimal growth conditions were obtained is that the ventilation interval was 24 h,light intensity was 20μmol/(m^2·s),and the ratio of mixed gas(CH4:O2:CO2)was 2:2:1(V:V).Under the optimal growth condition,the lag phase(λ)was 15.37 h,the maximum specific growth rate(μmax)was 0.0031 h-1,and the dry weight of the single cell protein per 100 mL of fermentation broth was 0.173 g.Finally,the effects of several metal ions(Cu^2+,Mg^2+,Fe^3+)on cell growth in co-culture systems were discussed.It was indicated that the co-cultivation of methanotrophs system could significantly promote the growth of methanotrophs,shorten the fermentation cycle,and provided a reference for the realization of large-scale industrial production of SCP.

关 键 词：单细胞蛋白 甲烷氧化菌 光合细菌 共培养体系 

分 类 号：S816.3[农业科学—饲料科学]