我国中东部地区诺如病毒GⅠ.1和GⅡ.4型人群血清抗体水平研究  

Serum antibodies against norovirus GⅠ.1 and GⅡ.4 in populations in central and eastern China

作　　者：韩子泊 康赟 雷泽华 袁润余[2] 魏真妮 申硕 孙振璐[4] 唐芳 侯俊伟 杜丽芳 张靖 李启明 Han Zibo;Kang Yun;Lei Zehua;Yuan Runyu;Wei Zhenni;Shen Shuo;Sun Zhenlu;Tang Fang;Hou Junwei;Du Lifang;Zhang Jing;Li Qiming(The Sixth Laboratory,National Vaccine&Serum Institute,Beijing 101111,China;Laboratory of Guangdong Public Health Institute,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430,China;The First Laboratory of Viral Vaccines,Wuhan Institute of Biological Products Co.,Ltd,Wuhan 430070,China;Microbiology Laboratory,Yantai Municipal Center for Disease Control and Prevention,Yantai 264003,China)

机构地区：[1]国药中生生物技术研究院有限公司第六研究室,北京101111 [2]广东省疾病预防控制中心,广东省公共卫生研究院实验室,广州511430 [3]武汉生物制品研究所有限责任公司病毒性疫苗研究一室,430070 [4]山东省烟台市疾病预防控制中心微生物检验科,264003

出　　处：《中华微生物学和免疫学杂志》2019年第11期840-847,共8页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究我国人群中诺如病毒(norovirus,NoV)GⅠ.1和GⅡ.4型特异性IgG、IgA和组织血型抗原(histo-blood group antigen,HBGA)阻断抗体水平,从血清学角度认识我国诺如病毒流行情况,为疫苗研发、临床实验和疫苗免疫策略的设计提供数据参考。方法采用间接ELISA法和HBGA受体阻断试验,对采集自广州、襄阳和烟台的839份自然人群(年龄跨度为6月龄至88周岁)血清中NoV特异性IgG、IgA和HBGA阻断抗体进行检测并对检测结果进行统计学分析。结果人群NoV GⅠ.1和GⅡ.4型特异性IgG抗体总阳性率为91.9%和93.0%,IgA抗体总阳性率为48.6%和75.6%,HBGA阻断抗体效价分别为5.04(95%置信区间:4.63~5.49)和18.15(95%置信区间:16.11~20.44)。IgG和IgA抗体阳性率均随年龄呈上升趋势。各年龄组GⅠ.1和GⅡ.4特异性IgG抗体阳性率范围分别为79.2%~100.0%和76.7%~100.0%,其中0.5~<1岁为81.7%和85.0%,1~<2岁为79.2%和76.7%,至12~<18岁增加至98.1%和96.3%,其后分别维持在96%和98%以上。各年龄组GⅠ.1特异性IgA抗体阳性率范围为11.7%~93.8%,其中0.5~<1岁为11.7%,随年龄增加快速上升,至45~<60岁和≥60岁达到93.3%和93.8%;GⅡ.4型特异性IgA抗体阳性率范围为50.8%~88.8%,其中0.5~<1岁为50.8%,12~<18岁开始达到并保持在86.7%~90.7%。GⅠ.1型HBGA阻断抗体效价总体呈随年龄上升趋势,其中0.5~<12岁各年龄组抗体效价显著低于18岁及以上各年龄组(GMT:2.98~4.07 vs 8.21~11.62,P<0.001),12~<18岁年龄组抗体效价显著低于45岁及以上各年龄组(GMT:5.21 vs 11.03~11.62,P<0.05),差异均有统计学意义;GⅡ.4型HBGA阻断抗体效价未见明显的随年龄变化趋势,仅在2~<5岁和22~<45岁人群间具有统计学差异(GMT:26.73 vs 11.87,P<0.01)。结论获得了我国中东部地区人群血清中NoV GⅠ.1和GⅡ.4型特异性IgG、IgA和HBGA阻断抗体数据,揭示了其在不同年龄组的分布规律,为我国NoV疫苗的研发提供了初步的血清流行病学资料。Objective To detect norovirus(NoV)GⅠ.1-and GⅡ.4-specific IgG,IgA and histo-blood group antigen(HBGA)-blocking antibodies in healthy populations of all age groups in China for better understanding the epidemiological features of norovirus in China from a serological point of view and providing basic data for vaccine development and clinical trial design.Methods Indirect ELISA and HBGA-blocking assay were used to detect NoV-specific IgG,IgA and HBGA-blocking antibodies in serum samples collected from healthy natural populations(n=839,aged from six months to 88 years old)in Guangzhou,Fuyang and Yantai.The results were statistically analyzed.Results The total positive rates of NoV GⅠ.1-and GⅡ.4-specific IgG antibodies were 91.9%and 93.0%.The positive rates of GⅠ.1-and GⅡ.4-specific IgA antibodies were 48.6%and 75.6%,and the titers of HBGA-blocking antibodies to GⅠ.1 and GⅡ.4 norovirus were 5.04(95%CI:4.63-5.49)and 18.15(95%CI:16.11-20.44).The positive rates of IgG and IgA antibodies generally showed an increasing trend with age.The positive rates of GⅠ.1-and GⅡ.4-specific IgG antibodies ranged from 79.2%to 100.0%and 76.7%to 100.0%in different age groups.They were 81.7%and 85.0%in the age group of 0.5-<1 year,79.2%and 76.7%in the age group of 1-<2 years,and 98.1%and 96.3%in the age group of 12-<18 years,and maintained at 96%and 98%in the older age groups.The positive rates of GⅠ.1-specific IgA antibody ranged from 11.7%to 93.8%in different age groups and rapidly increased with age.It was 11.7%in the age group of 0.5-<1 year,and reached 93.3%in people aged 45-<60 years and 93.8%in people aged≥60 years.The positive rates of GⅡ.4-specific IgA antibody ranged from 50.8%to 88.8%in different age groups with 50.8%in people aged 0.5-<1 year,and 86.7%-90.7%in people aged 12-<18 years and older.The titer of GⅠ.1 HBGA-blocking antibody generally increased with age.The antibody titer in populations aged 0.5-<12 years old was lower than that in those aged 18 years and above(GMT:2.98-4.07 vs 8.21-11.62,P

关 键 词：诺如病毒 血清抗体 受体阻断试验 

分 类 号：R73[医药卫生—肿瘤]