牛脑蛋白的碱法提取工艺及氨基酸组成分析  

Study on Alkaline Solution Extraction and Amino Acid Compositions Analysis of Bovine Brain Protein

作　　者：张建萍[1] 于楠楠 刘辉[1] 巫永华[1] 刘恩岐[1] ZHANG Jianping;YU Nannan;LIU Hui;WU Yonghua;LIU Enqi(Jiangsu Key Laboratory of Food Resource Development and Quality Safety,Xuzhou Institute of Technology,Xuzhou 221008)

机构地区：[1]徐州工程学院江苏省食品资源开发与质量安全重点建设实验室

出　　处：《食品工业》2019年第11期26-30,共5页The Food Industry

摘　　要：动物脑组织含有丰富的蛋白质,试验以冷冻干燥牛脑为原料,通过单因素试验和正交试验,研究碱性溶液提取牛脑蛋白的最佳工艺,并对牛脑蛋白的沉淀方法进行研究。结果表明,牛脑蛋白提取的最佳工艺条件为:料液比1︰40(g/mL)、氢氧化钠质量分数1.2%、提取温度60℃、提取时间3.5 h。此条件下牛脑蛋白的提取率为92.9%。选择等电点和盐析共同作用的沉淀方法,获得的牛脑蛋白沉淀率为90.8%,牛脑蛋白纯度为87.3%。氨基酸分析结果表明:牛脑蛋白含有17种氨基酸,其中必需氨基酸含量占22.16%。Animal brain tissue was a rich protein resource.Freeze-dried bovine brain was used as a raw material.The single factor experiment and orthogonal experiment were performed to study the best extraction process of bovine brain protein using alkaline solution.The precipitation method of bovine brain protein was also carried out.The results showed that the optimum extraction process of bovine brain protein was solid-liquid ratio of 1︰40(g/mL),the concentration of sodium hydroxide was 1.2%,the extraction temperature was 60℃,and the extraction time of 3.5 h.The extraction yield of bovine brain protein was 92.9%under this condition.The isoelectric point and salt-precipitation were selected as isoelectric point,and salt-precipitation was selected the precipitation method.The precipitation of bovine brain protein was 90.8%,and the purity of bovine brain protein was 87.3%.The analysis of amino acid compositions showed that the bovine brain protein contains 17 kinds of amino acids and 22.16%essential amino acids.

关 键 词：牛脑蛋白 提取 沉淀 氨基酸分析 

分 类 号：TS2[轻工技术与工程—食品科学与工程]