联合法体外分离培养牙周膜细胞的研究  

Combined Method for Isolation and Culture of Periodontal Ligament Cells in Vitro

作　　者：戚琳[1] 孙佳 原玉洁 赵迎帆 薛楠[1] QI Lin;SUN Jia;YUAN Yu-jie;ZHAO Ying-fan;XUE Nan(Department of Orthodontics,School and Hospital of Stomatology,China Medical University,Shenyang,Liaoning,110001,China)

机构地区：[1]中国医科大学口腔医学院,中国医科大学附属口腔医院正畸一科,辽宁沈阳110001

出　　处：《中国血液流变学杂志》2019年第2期151-154,204,共5页Chinese Journal of Hemorheology

基　　金：辽宁省博士科研启动基金(201601131)。

摘　　要：目的该研究采用将牙周膜细胞(periodontal ligament cells,PDLCs)培养中经典的组织块法和酶消化法结合起来的联合法进行PDLCs分离培养,旨在寻找一种简单易行的PDLCs培养模式,缩短牙周膜原代细胞培养周期.方法取25颗因正畸需要拔除的完整双尖牙,运用联合法进行牙周膜原代细胞的提取培养,计算成功率.结果采用联合法分离培养可获得PDLCs,所得的PDLCs呈长梭形,符合PDLCs的形态和生物学特征,长势良好,其成功率为40%.结论运用联合法提取培养PDLCs的方法可行,所得细胞形态及长势良好.Objective To explore a simple and practicable cultivating mode of periodontal ligament cells (PDLCs) to shorten the cultivating period of primary periodontal ligament cells. This study combined tissue cultivation of PDLCs with enzyme digestion and used this combination method to isolate and culture PDLCs. Methods Take 25 premolars which need to extract because of orthodontics, and then use combination method to extract and cultivate the primary periodontal ligament cells. The success rate was calculated. Results Combined method can isolate and cultivate primary periodontal ligament cells. The PDLCs is elongated, and conform to the form and biological characteristics of PDLCs. The PDLCs grow well and the success rate is 40%. Conclusion The way of using combination method to extract and cultivate PDLCs is practicable, and the form and growth of the PDLCs are well.

关 键 词：联合法 牙周膜细胞 原代培养 

分 类 号：R783.5[医药卫生—口腔医学]