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作 者:Petit Claudie Guignandon Alain Avril Stéphane
机构地区:[1]Mines Saint-Etienne,Universitéde Lyon,INSERM,U 1059 SAINBIOSE,F-42023 Saint-Etienne,France [2]UniversitéJean Monnet,Universitéde Lyon,INSERM,U 1059 SAINBIOSE,F-42023 Saint-Etienne,France
出 处:《Molecular & Cellular Biomechanics》2019年第2期87-108,共22页分子与细胞生物力学(英文)
摘 要:The contractile behavior of smooth muscle cells(SMCs)in the aorta is an important determinant of growth,remodeling,and homeostasis.However,quantitative values of SMC basal tone have never been characterized precisely on individual SMCs.Therefore,to address this lack,we developed an in vitro technique based on Traction Force Microscopy(TFM).Aortic SMCs from a human lineage at low passages(4-7)were cultured 2 days in conditions promoting the development of their contractile apparatus and seeded on hydrogels of varying elastic modulus(1,4,12 and 25 kPa)with embedded fluorescent microspheres.After complete adhesion,SMCs were artificially detached from the gel by trypsin treatment.The microbeads movement was tracked and the deformation fields were processed with a mechanical model,assuming linear elasticity,isotropic material,plane strain,to extract the traction forces formerly applied by individual SMCs on the gel.Two major interesting and original observations about SMC traction forces were deduced from the obtained results:1.they are variable but driven by cell dynamics and show an exponential distribution,with 40%to 80%of traction forces in the range 0-10μN.2.They depend on the substrate stiffness:the fraction of adhesion forces below 10μN tend to decrease when the substrate stiffness increases,whereas the fraction of higher adhesion forces increases.As these two aspects of cell adhesion(variability and stiffness dependence)and the distribution of their traction forces can be predicted by the probabilistic motor-clutch model,we conclude that this model could be applied to SMCs.Further studies will consider stimulated contractility and primary culture of cells extracted from aneurysmal human aortic tissue.
关 键 词:Cell biomechanics aortic aneurysm MECHANOTRANSDUCTION MECHANOSENSITIVITY smooth muscle cells(SMC) fluorescent microscopy(FM) traction force microscopy(TFM) isolated cells
分 类 号:TG1[金属学及工艺—金属学]
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