DNA倍体分析联合血清肿瘤标志物检测在宫颈癌筛查中的应用  

Value of DNA ploidy analysis combined with serum oncology index detection in early cervical cancer screening

作　　者：徐曼[1] 蒋艳[1] 朱燕燕[1] 杨小苗[1] 申成香 怀建国[1] XU Man;JIANG Yan;ZHU Yanyan;YANG Xiaom iao;SHEN Chengxiang;HUAI Jianguo(Department of Pathology,First People's Hospital of Wuhu,Wuhu,Anhui 241000,China)

机构地区：[1]安徽省芜湖市第一人民医院病理科

出　　处：《检验医学与临床》2019年第24期3560-3562,共3页Laboratory Medicine and Clinic

基　　金：卫生部医药卫生科技发展研究中心课题（W2013GJ44）

摘　　要：目的探讨DNA倍体分析联合血清肿瘤标志物在宫颈癌筛查中的应用价值。方法选择2017年9月至2018年9月于该院进行宫颈癌筛查的350例患者为研究对象,采用DNA倍体分析技术对患者进行诊断,同时检测患者糖类抗原125(CA-125)、癌胚抗原(CEA),并进行病理组织检查。比较DNA倍体分析、CA-125、CEA水平对宫颈癌的诊断价值。结果DNA倍体分析诊断宫颈癌的阳性率与病理组织检出的阳性率差异有统计学意义(χ~2=15.810,P<0.001);宫颈癌患者血清CA-125、CEA水平显著高于病理检查阴性或宫颈炎、宫颈上皮内瘤变(CIN)患者(P<0.05);受试者工作特征曲线(ROC曲线)分析结果显示,DNA倍体分析联合血清CA-125、CEA水平诊断宫颈癌的曲线下面积(AUC)为0.906,明显高于DNA倍体分析、CA-125、CEA单独检测(P<0.05)。结论DNA倍体分析联合血清CA-125、CEA进行检测可以提高宫颈癌的阳性检出率,值得临床推广。Objective To investigate the value of DNA ploidy analysis combined with serum oncology index in early cervical cancer screening.Methods A total of 350 patients who underwent cervical cancer lesions screening in the hospital from September 2017 to September 2018 were collected.DNA ploidy analysis was used to diagnose the patients and serum oncology index were performed to conduct examination.At the same time,carbohydrate antigen 125(CA-125),carcinoembryonic antigen(CEA)were detected,the pathological tissue was detected too.Diagnostic value of DNA ploidy analysis and serum oncology indexes such as CA-125 and CEA for cervical cancer were compared.Results The test results of DNA ploidy analysis showed that positive rate was statistically different from that of pathological tissue detection(χ^2=15.810,P<0.001).The test results of serum oncology indexes showed levels of serum CA-125 and CEA in cervical cancer patients were significantly higher than those in patients with negative results by pathological examination,cervicitis and cervical intraepithelial neoplasia(CIN)patients(P<0.05).The analysis results of areas under ROC curve(AUC)showed that AUC of DNA ploidy analysis combined with serum CA-125 and CEA levels for diagnosis and prediction of cervical cancer was 0.906,significantly higher than that of DNA ploidy analysis,CA-125 and CEA alone(P<0.05).Conclusion DNA ploidy analysis combined with serum CA-125 and CEA levels could improve predictive rate of early screening for cervical cancer,which is worth of popularization.

关 键 词：宫颈癌 DNA倍体分析 癌胚抗原 糖类抗原125 

分 类 号：R362[医药卫生—病理学]