Effects of four components from Uncaria rhynchophylla on LTP impairment induced by lipopolysaccharide  

作　　者：SUN Na WANG Chen HUANG Yan ZHOU Wen-xia ZHAO Qin-shi 

机构地区：[1]Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China [2]State Key Laboratory of Toxicology and Medical Countermeasures,Beijing 100850,China [3]State Key Laboratory of Phytochemistry and Sustainable Utilization of Plant Resources in Western China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

出　　处：《中国药理学与毒理学杂志》2019年第9期737-737,共1页Chinese Journal of Pharmacology and Toxicology

基　　金：Open Fund from the State Key Laboratory of Phytochemistry and Plant Resources in West China(P2017-KF13);National Science and Technology Major Project of China(2016ZX09J16104)

摘　　要：OBJECTIVE To evaluate the neuroprotective effects of 4 components from Uncaria rhynchophylla(297,307,315 and 327)on long-term potentiation(LTP)deficit in neuroinflammation animal model induced by lipopolysaccha⁃ride(LPS).METHODS Male BALB/c 18-22 g mice were divided into control group,model group and component treat⁃ment group(1μg per mouse,icv);each group contained 5 mice,model group and compound treatment group were intra⁃peritoneally injected LPS(50μg·kg^-1,ip)4 h before LTP induction.LTP of perforant path-dentate gyrus pathway in hippo⁃campus was induced by high frequency stimulation and used to evaluate the effects on synaptic plasticity.RESULTS Compared with control group,the LTP of the model group was significantly impaired.Compound 297,327,and 307 could significantly improve LPS-induced LTP impairment.315 had no significant effect on LPS-induced LTP impairment.CONCLUSION Hippocampal synaptic plasticity could be impaired by LPS.Compounds 297,327 and 307 have protec⁃tive effects against LPS induced LTP impairment,and 315 has little effect on LPS induced LTP impairment.These re⁃sults suggested that 297,327 and 307 might have potential effects on neuroinflammation induced memory deficits.OBJECTIVE To evaluate the neuroprotective effects of 4 components from Uncaria rhynchophylla（297,307, 315 and 327） on long-term potentiation（LTP） deficit in neuroinflammation animal model induced by lipopolysaccharide（LPS）. METHODS Male BALB/c 18-22 g mice were divided into control group, model group and component treatment group（1 μg per mouse, icv）; each group contained 5 mice, model group and compound treatment group were intraperitoneally injected LPS(50 μg·kg-1, ip) 4 h before LTP induction. LTP of perforant path-dentate gyrus pathway in hippocampus was induced by high frequency stimulation and used to evaluate the effects on synaptic plasticity. RESULTS Compared with control group, the LTP of the model group was significantly impaired. Compound 297, 327, and 307 could significantly improve LPS-induced LTP impairment. 315 had no significant effect on LPS-induced LTP impairment.CONCLUSION Hippocampal synaptic plasticity could be impaired by LPS. Compounds 297, 327 and 307 have protective effects against LPS induced LTP impairment, and 315 has little effect on LPS induced LTP impairment. These results suggested that 297, 327 and 307 might have potential effects on neuroinflammation induced memory deficits.

关 键 词：LIPOPOLYSACCHARIDE HIPPOCAMPAL long-term potentiation 

分 类 号：R33[医药卫生—人体生理学]