厚褶小奥德蘑栽培及其抗氧化活性  

Cultivation and Antioxidant Activity of Oudemansiella crassifolia

作　　者：史钏 胡惠萍[1] 莫伟鹏 刘远超[1] 谢意珍 SHI huan;HU Huiping;MO Weipeng;LIU Yuanchao;XIE Yizhen(Guangdong Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Colection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangzhou,Guangdong 510070,China;Yuewei Edible Fungi Technology Co.Ltd.,Guangzhou,Guangdong 510663,China)

机构地区：[1]广东省微生物研究所广东省科学院,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510075 [2]广东粤微食用菌技术有限公司,广东广州510663

出　　处：《食用菌学报》2019年第4期78-83,共6页Acta Edulis Fungi

基　　金：广东省重点领域研发计划项目(2018B020205001)

摘　　要：对采自广东省鼎湖山的一株野生小奥德蘑(Oudemansiella sp.)进行分类鉴定与栽培,研究其液体培养菌丝体的水提液、醇提液和发酵液的抗氧化活性。采用形态学特征与rDNA-ITS序列分析相结合的方法,将其鉴定为厚褶小奥德蘑(O.crassifolia)。利用栽培料(58%杂木屑、30%棉籽壳、10%麸皮、2%碳酸钙,含水量65%,pH自然),在(25±1)℃条件下遮光培养20 d,菌丝满袋后再培养15~16 d,转入出菇房,在25~28℃、相对湿度85%~90%条件下,每日300~500 lx光照10 h,菇蕾产生后,每日通风3~4次,出菇3~4潮,生物学转化率达到79.15%。本研究中,厚褶小奥德蘑菌丝体水提液的还原力和对羟自由基的清除能力明显高于菌丝体醇提液和发酵液,与对照维生素C相比,厚褶小奥德蘑菌丝体水提液的还原力低于维生素C,而对羟自由基的清除能力是维生素C的1.81倍。A wild strain of Oudemansiella sp.was collected from Dinghushan national nature reserve in Guangdong Province and identified as O.crassifolia based on morphological observations and rDNA-ITS sequence analysis.Then this strain was studied for artificial cultivation and the antioxidant activity of its water extract,ethanol extract and mycelial fermentation broth,respectively.The results showed that this strain of O.crassifolia was successfully cultivated on a substrate comprising 58%wood chips,30%cottonseed hull,10%wheat bran,and 2%calcium carbonate with 65%moisture and natural pH.The cultivation was first carried out in shade at(25±1)℃for 20 d to allow full growth of mycelia in cultivation bags.Then the bags were incubated under the same condition for another 15-16 d prior to transference to the mushroom chamber for further development of mushroom buds under 25-28℃,relative humidity 85%-90%,and 300-500 lx light for 10 h each day.Upon development of mushroom buds,the mushroom chamber was ventilated 3-4 times a day for fruit body maturation.The mature fruit bodies were collected for 3-4 flushes and the overall biological efficiency was 79.15%.Among all tested samples,the reducing power and hydroxyl radical scavenging ability of water extract of O.crassifolia mycelia were significantly higher than that of mycelium ethanol extract and fermentation broth.Compared with vitamin C,water extract of O.crassifolia mycelia showed lower reducing power.On the other hand,its hydroxyl radical scavenging ability was 1.81 times of vitamin C.

关 键 词：小奥德蘑属 ITS分析 栽培 还原力 羟自由基 

分 类 号：S64[农业科学—蔬菜学]