多花黄精内生真菌Aspergillus ochraceus的代谢产物研究  

Study on secondary metabolites of endophytic fungus Aspergillus ochraceus from Polygonatum cyrtonema

作　　者：程子洋[1] 柯仲成[1] 吴永祥[2] CHENG Zi-yang;KE Zhong-cheng;WU Yong-xiang(College of Chemistry and Chemical Engineering,Huangshan University,Huangshan 245041,China;College of Life and Environment Science,Huangshan University,Huangshan 245041,China)

机构地区：[1]黄山学院化学化工学院,安徽黄山245041 [2]黄山学院生命与环境科学学院,安徽黄山245041

出　　处：《中草药》2019年第22期5424-5428,共5页Chinese Traditional and Herbal Drugs

基　　金：安徽省高校自然科学一般研究项目(KJHS2019B05);安徽省留学回国人员创新项目择优资助计划重点项目(2017srst1);安徽省高校优秀青年骨干人才国内访问研究项目(gxgnfx2019037);博士人才引进启动基金项目(2016xkjq004)

摘　　要：目的研究多花黄精内生真菌Aspergillus ochraceus的次生代谢产物。方法利用硅胶、SephadexLH-20、中压及高效液相制备等多种柱色谱法分离化合物,根据谱学方法对化合物进行结构鉴定。结果从内生真菌Aspergillus ochraceus中分离得到15个化合物,分别鉴定为6,7-dihydroindolizin-8(5H)-one(1)、polygonatine A(2)、8-oxo-5,6,7,8-tetrahydro-3-indolizinyl methyl acetate(3)、8-羟基色原酮(4)、环(L-亮-L-异亮)二肽(5)、交链孢酚(6)、seco-patulolide C(7)、正丁基-β-D-呋喃果糖苷(8)、Nb-乙酰色胺(9)、N-反式-桂皮酰酪胺(10)、5-羟甲基-2-呋喃糠醛(11)、5,7-二羟基-6,8-二甲基-3-(4′-羟基苯基)色烷-4-酮(12)、5,7-二羟基-6-甲基-8-甲氧基-3-(4′-羟基苯基)色烷-4-酮(13)、25R-3β-羟基螺甾-5-烯-12-酮(14)、25S-3β-羟基螺甾-5-烯-12-酮(15)。结论所有化合物均首次从内生真菌Aspergillus ochraceus中分离得到。Objective To investigate the secondary metabolites of Aspergillus ochraceus,an endophytic fungus isolated from Polygonatum Cyrtonema.Methods Compounds were isolated and purified from the EtOAc extract by using chromatography technology and their structures were established on the basis of comprehensive spectroscopic analysis.Results A total of 15 compounds were obtained and their structures were elucidated as 6,7-dihydroindolizin-8(5H)-one(1),polygonatine A(2),8-oxo-5,6,7,8-tetrahydro-3-indolizinyl methyl acetate(3),8-hydroxyketone(4),cyclo-(L-Leu-L-Ile)(5),alternariol(6),secopatulolide C(7),n-butyl-β-D-fructopyranoside(8),Nb-acetyltryptamine(9),N-trans-cinnamoyltyramine(10),5-hydroxymethylfurfural(11),5,7-dihydroxy-6,8-dimethyl-3-(4′-hydroxybenzyl)chroman-4-one(12),5,7-dihydroxy-6-methyl-8-methoxy-3-(4′-hydroxybenzyl)chroman-4-one(13),25R-3-β-hydroxyspirost-5-en-12-one(14),and 25S-3-β-hydroxyspirost-5-en-12-one(15).Conclusion All chemical constituents are isolated from A.ochraceus for the first time.

关 键 词：多花黄精 Aspergillus ochraceus 内生真菌 次生代谢产物 8-羟基色原酮 环(L-亮-L-异亮)二肽 交链孢酚 

分 类 号：R284.1[医药卫生—中药学]