人参皂苷Rg1对小鼠非酒精性脂肪肝的改善作用和机制研究  

Effect of ginsenoside Rg1 against non-alcoholic fatty liver disease in mice and its mechanism

作　　者：徐雅姝 黄文祥[1] 阳成[1] 章述军[1] 赵罗乐 秦圆圆 Xu Yashu;Huang Wenxiang;Yang Cheng;Zhang Shujun;Zhao Luole;Qin Yuanyuan(Department of Infectious Diseases,The First Affiliated Hospital of Chongqing Medical University)

机构地区：[1]重庆医科大学附属第一医院感染科

出　　处：《重庆医科大学学报》2019年第11期1434-1438,共5页Journal of Chongqing Medical University

基　　金：重庆市科委基础科学与前沿技术研究重点资助项目（编号：CSTC2015jcyjBX0079）

摘　　要：目的:探讨人参皂苷(ginsenoside,Rg1)对高脂饮食诱导小鼠非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的改善作用及可能的机制。方法:将42只健康成年C57/BL雌性小鼠随机分为对照组、模型组、Rg1低剂量组、Rg1高剂量组、二甲双胍组,对照组和模型组每组各9只小鼠,其余小组每组各8只小鼠。对照组给予普通饲料喂养,其余各组给予高脂饲料喂养,持续喂养16周。16周后,对照组和模型组给予0.9%生理盐水20 mL/(kg·d)灌胃,Rg1低剂量组给予人参皂苷Rg120 mg/(kg·d)灌胃,Rg1高剂量组给予人参皂苷Rg140 mg/(kg·d)灌胃,二甲双胍组给予二甲双胍150 mg/(kg·d)灌胃。持续治疗4周后采集各组小鼠血清和肝脏组织。观察各组肝组织病理学形态,测定血清转氨酶及血脂水平、肝组织匀浆中丙二醇(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、游离脂肪酸(free fatty acid,FFA)的含量及内质网应激(endoplasmic reticulum stress,ERS)与炎症小体相关蛋白的表达情况。结果:Rg1低、高剂量组血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)分别为(41.87±10.64)、(43.46±13.84)U/L,天冬氨酸氨基转移酶(aspartate aminotransferase,AST)分别为(159.56±21.29)、(159.56±25.01)U/L,甘油三酯(triglyceride,TG)分别为(0.69±0.15)、(0.75±0.12)U/L,均明显低于模型组(P=0.001,P=0.010,P=0.000),肝组织脂肪变性程度明显轻于模型组(P=0.000)。Rg1能降低FFA,MDA含量,提高SOD活力(均P=0.000)。Rg1能下调GRP78、CHOP、Caspase12、NLRP3、IL-1β蛋白的表达(P=0.000,P=0.003)。结论:Rg1可能通过提高抗氧化酶活性、抑制ERS相关分子及炎症小体活化来改善NAFLD。Objective:To investigate the effect of ginsenoside Rg1 on non-alcoholic fatty liver disease(NAFLD)in mice induced by high fat diet and its possible mechanism.Methods:A total of 42 healthy female adult C57/BL mice were randomly divided into the control group,model group,Rg1 low dose group,Rg1 high dose group,and metformin group,with 9 mice in each of the previous two groups and 8 in each of the latter.The control group was fed with the normal diet,while the others were fed with the high fat diet,and the feeding was continued for 16 weeks.Then the mice were treated with gastric perfusion,among them,the control group and the model group were treated with normal saline 20 mL(kg·d),Rg1 low dose group with Rg120 mg/(kg·d),Rg1 high dose group with Rg140 mg/(kg·d),and metformin group with Rg1150 mg/(kg·d).After 4 weeks of treatment,the serum and liver tissues of each group were collected.The pathological morphology of liver tissue was observed.The serum transaminase,lipid levels,the content of malondialdehyde(MDA),superoxide dismutase(SOD),free fatty acids(FFA)and the expression of endoplasmic reticulum stress(ERS)related proteins and inflammasome were measured.Results:In Rg1 low dose group and high dose group,the levels of serum alanine aminotransferase(ALT)were(41.87±10.64)and(43.46±13.84)U/L,the levels of aspartate aminotransferase(AST)were(159.56±21.29)and(159.56±25.01)U/L,and the levels of triglycerides(TG)were(0.69±0.15)and(0.75±0.12)U/L respectively,all of which were significantly lower than those in the model group(P=0.001,P=0.010,P=0.000).And the fatty degeneration degree of liver tissue in the two groups was also significantly smaller than that in the model group(P=0.000).Rg1 could reduce the contents of FFA and MDA,increase the vitality of SOD(all P=0.000),and down-regulate the expressions of GRP78,CHOP,Caspase12,NLRP3 and IL-1β(P=0.000,P=0.003).Rg1 can also reduce the expression of endoplasmic reticulum stress related proteins and inflammasome activation(P=0.000,P=0.003).Conclusion:Rg1 may improve

关 键 词：非酒精性脂肪肝 人参皂苷RG1 氧化应激 内质网应激 炎症小体 

分 类 号：R575.5[医药卫生—消化系统]