马铃薯多酚氧化酶基因家族生物信息学及表达分析  

Bioinformatics and expression analysis of polyphenol oxidase gene family in potato

作　　者：王丽[1,2,3] 王万兴[4,5] 索海翠 胡新喜[1] 秦玉芝 李小波[2,3] 熊兴耀 WANG Li;WANG Wanxing;SUO Haicui;HU Xinxi;QIN Yuzhi;LI Xiaobo;XIONG Xingyao(College of Horticulture and Landscape,Hunan Agricultural University,Changsha,Hunan 410128,China;Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China;Provincial Key Laboratory of Crops Genetic Improvement,Guangzhou,Guangdong 510640,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081,China;Key Laboratory of Biology and Genetic Improvement of Tuber Crops of the Ministry of Agriculture,Beijing,100081,China)

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]广东省农业科学院作物研究所,广东广州510640 [3]广东省农作物遗传改良重点实验室,广东广州510640 [4]中国农业科学院蔬菜花卉研究所,北京100081 [5]农业部薯类作物生物学与遗传育种重点实验室,北京100081

出　　处：《湖南农业大学学报（自然科学版）》2019年第6期601-610,共10页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家公益性行业(农业)科研专项(201503001);广东省省级科技计划项目(2017A020208033,2017B020201005,2017B020232002);广东省自然科学基金项目(2018A030313336);2017年省级农业发展和农村工作专项(粤财农[2017]88号);中国农业科学院科技创新项目(CAAS–ASTIP–IVFCAAS);作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目(18KFXM03)

摘　　要：通过搜索马铃薯全基因组,获得马铃薯多酚氧化酶(PPO)基因家族序列,并对其进行生物信息学及表达分析。结果显示:共获得12个马铃薯PPO基因,即StuPPO1、StuPPO2、StuPPO3、⋯、StuPPO12;除StuPPO9位于2号染色体外,其余11个基因串联于8号染色体的276 kb范围内;对具有3个典型结构域(Tyrosinase、PPO1_DWL、PPO1_KFDV)的基因蛋白序列进行进一步分析,结果显示StuPPO1、StuPPO2、StuPPO3、StuPPO4、StuPPO6蛋白序列相似性大于80%,且大部分StuPPO蛋白位于叶绿体;启动子顺式作用元件分析显示,StuPPO1、StuPPO2、StuPPO3、StuPPO6、StuPPO8、StuPPO9具有MeJA响应元件,StuPPO1、StuPPO3、StuPPO4、StuPPO6、StuPPO7、StuPPO8、StuPPO9具有ABA响应元件,StuPPO2、StuPPO4、StuPPO8、StuPPO9具有IAA响应元件;少数PPO基因具有对SA、GA及低温等胁迫响应相关元件;基因表达分析结果表明,不同的PPO基因其表达模式有较大的差异,StuPPO1、StuPPO2、StuPPO3在马铃薯块茎、根、匍匐茎、茎、叶柄、顶部幼叶、中部叶片、底部老叶组织中均有表达,StuPPO4仅在块茎、根、匍匐茎、茎中表达,而StuPPO6、StuPPO7、StuPPO8、StuPPO9在8个部位都没有检测到表达。此外,同一基因在不同组织中的表达也有着较大的差异。The sequences of the polyphenol oxidase(PPO)gene family were searched from potato genome and were subject to bioinformatics and expression analysis.The results showed that a total of 12 PPO genes,StuPPO1,StuPPO2,StuPPO3,⋯,StuPPO12 with exception of StuPPO9 localized on chromosome 2,were sorted out and all of them located in the 276 kb range of chromosome 8.Further analysis of the PPO protein sequences with 3 typical structural domains(Tyrosinase,PPO1_DWL,PPO1_KFDV)showed that the similarity of StuPPO1,StuPPO2,StuPPO3,StuPPO4 and StuPPO6 protein sequences was more than 80%,and most of the StuPPO proteins are in chloroplasts.The analysis of cis-acting elements of the promoters showed that StuPPO1,StuPPO2,StuPPO3,StuPPO6,StuPPO8 and StuPPO9 have elements responsive to MeJA;StuPPO1,StuPPO3,StuPPO4,StuPPO6,StuPPO7,StuPPO8,StuPPO9 have element responsive to ABA;StuPPO2,StuPPO4,StuPPO8,StuPPO9 have elements responsive to IAA;A few PPO genes have elements responsive to SA,GA and low temperature.The results of gene expression analysis showed that the expression patterns of different PPO genes of potato were significantly different.StuPPO1,StuPPO2 and StuPPO3 were expressed in the tissues of potato tuber,root,stolon,stem,petiole,young leaves,middle leaves and old leaves;StuPPO4 is only expressed in the tissues of tuber,root,stolon and stem;StuPPO6,StuPPO7,StuPPO8 and StuPPO9 were not expressed in any tissues tested.In addition,the expression of the same gene varied greatly in different tissues of potato.

关 键 词：马铃薯 PPO基因家族 生物信息学 基因表达 

分 类 号：S532[农业科学—作物学] Q786[生物学—分子生物学]