Chromosome doubling of Sedum alfredii Hance: A novel approach for improving phytoremediation efficiency  

作　　者：Ying Feng Qiong Wang Qian Meng Yongjun Liu Fengshan Pan Sha Luo Yingjie Wu Luyao Ma Xiaoe Yang 

机构地区：[1]MOE Key Laboratory of Environment Remediation and Ecological Health,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China [2]Hunan Tobacco Scientific Research Institute,China National Tobacco Corp Company,Changsha 410004,China [3]Hailiang Group Co.,Ltd.,Hangzhou 310058,China

出　　处：《Journal of Environmental Sciences》2019年第12期87-96,共10页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Project of China (No. 2016YFD0800801);the National Natural Science Foundation of China (No. 41771345);the Fundamental Research Funds for the Central Universities (2019FZJD007)

摘　　要：Sedum alfredii Hance is a cadmium(Cd)/zinc(Zn)hyperaccumulator native to China.However,its relatively low biomass restricted the large-scale application for heavy metal contamination remediation.The chromosome set doubling of S.alfredii in vitro was achieved by 0.1%–0.2%(W/V)colchicine treatment.The plant DNA ploidy was analyzed by flow cytometry and chromosome set doubling plants(CSD)were identified based on the obvious different sharp peak.A tissue culture experiment with different Cd treated levels and a field trial with natural polluted mined soil were conducted to study the effects of chromosome doubling on plant biomass and Cd accumulation in shoots.The results suggested that S.alfredii is a mixoploid.Compared with the wild type plants(WT),CSD exhibited typical"gigas"characteristics in morphology including stem thickness,root hair production,number of leaves and size of stoma guard cell.Fresh weight and dry weight of CSD were increased to 1.62–2.03-fold and 2.26–3.25-fold of WT.And Cd content of CSD showed a17.49%–42.82%increase and 59%increase under tissue culture and field condition,accordingly.In addition,the TF and in BCF of CSD were 2.37-and 1.59-fold of WT,respectively.These results proved that it is feasible to promote phytoextraction efficiency of S.alfredii in Cd contaminated soils through chromosomal engineering,which provides a novel approach for hyperaccumulator application in phytoremediation.Sedum alfredii Hance is a cadmium(Cd)/zinc(Zn) hyperaccumulator native to China.However, its relatively low biomass restricted the large-scale application for heavy metal contamination remediation. The chromosome set doubling of S. alfredii in vitro was achieved by 0.1%–0.2%(W/V) colchicine treatment. The plant DNA ploidy was analyzed by flow cytometry and chromosome set doubling plants(CSD) were identified based on the obvious different sharp peak. A tissue culture experiment with different Cd treated levels and a field trial with natural polluted mined soil were conducted to study the effects of chromosome doubling on plant biomass and Cd accumulation in shoots. The results suggested that S.alfredii is a mixoploid. Compared with the wild type plants(WT), CSD exhibited typical"gigas" characteristics in morphology including stem thickness, root hair production,number of leaves and size of stoma guard cell. Fresh weight and dry weight of CSD were increased to 1.62–2.03-fold and 2.26–3.25-fold of WT. And Cd content of CSD showed a17.49%–42.82% increase and 59% increase under tissue culture and field condition,accordingly. In addition, the TF and in BCF of CSD were 2.37-and 1.59-fold of WT,respectively. These results proved that it is feasible to promote phytoextraction efficiency of S. alfredii in Cd contaminated soils through chromosomal engineering, which provides a novel approach for hyperaccumulator application in phytoremediation.

关 键 词：COLCHICINE HYPERACCUMULATOR Cd Sedum alfredii Hance PHYTOREMEDIATION 

分 类 号：Q94[生物学—植物学]