Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon  

作　　者：Quanhui Ye Chengyue Liang Xunwen Chen Tingting Fang Yun Wang Hui Wang 

机构地区：[1]State Key Joint Laboratory on Enuironment Simulation and Pollution Control,School o/Enuironment,Tsinghua University,Beijing 100084,China [2]Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology of China,Shenzhen 518055,China [3]State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Journal of Environmental Sciences》2019年第12期97-106,共10页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation Projects (Nos. 41573065 and 41773082);the Key Project of Natural Science Foundation of China (No. 21337001);the National Water Pollution Control and Treatment Science and Technology Major Project (No. 2017ZX07202002)

摘　　要：Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons(PAHs)is crucial to developing strategies for PAHs bioremediation.In this study,the linkage between the type of PAHs and microbial community structure was fully investigated through 16 S rRNA gene sequencing on four PAH-degrading cultures.Putative degradation products were also detected.Our results indicated that naphthalene(Nap)/2-methylnaphthalene(2-Nap),phenanthrene(Phe)and anthracene(Ant)sculpted different microbial communities.Among them,Nap and 2-Nap selected for similar degrading bacteria(i.e.,Alicycliphilus and Thauera)and methanogens(Methanomethylovorans and Methanobacterium).Nap and 2-Nap were probably activated via carboxylation,producing 2-naphthoic acid.In contrast,Phe and Ant shaped different bacterial and archaeal communities,with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader.Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture,respectively.These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons(PAHs) is crucial to developing strategies for PAHs bioremediation. In this study, the linkage between the type of PAHs and microbial community structure was fully investigated through 16 S rRNA gene sequencing on four PAH-degrading cultures. Putative degradation products were also detected. Our results indicated that naphthalene(Nap)/2-methylnaphthalene(2-Nap), phenanthrene(Phe) and anthracene(Ant)sculpted different microbial communities. Among them, Nap and 2-Nap selected for similar degrading bacteria(i.e., Alicycliphilus and Thauera) and methanogens(Methanomethylovorans and Methanobacterium). Nap and 2-Nap were probably activated via carboxylation, producing 2-naphthoic acid. In contrast, Phe and Ant shaped different bacterial and archaeal communities,with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader. Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture, respectively. These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.

关 键 词：Polycyclic aromatic hydrocarbons Microbial community structure Degradation products Degrading bacteria CARBOXYLATION 

分 类 号：S15[农业科学—土壤学]