胃癌中lncRNA-BBOX1-2、FGFR1的表达和意义  

Expressions and Significance of LncRNA-BBOX1-2 and FGFR1 in Gastric Cancer

作　　者：孙颖[1] 顾玮[1] 郑雄[1] 胡梅洁[2] 高天[3] SUN Ying;GU Wei;ZHENG Xiong;HU Meijie;GAO Tian(Department of Gastroenterology,Luwan Branch,Ruijin Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,200020;Department of Gastroenterology,Ruijin Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai;Department of Geriatrics,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai)

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院消化内科,200020 [2]上海交通大学医学院附属瑞金医院消化内科 [3]上海交通大学医学院附属仁济医院老年病科

出　　处：《胃肠病学》2019年第10期591-596,共6页Chinese Journal of Gastroenterology

基　　金：上海市卫计委科研课题(20154Y0208)

摘　　要：背景:多种肿瘤组织中存在长链非编码RNA(lncRNAs)表达失调。前期研究显示lncRNA-BBOX1-2在胃癌中的表达明显升高,而成纤维细胞生长因子受体1(FGFR1)为其可能的靶基因。目的:探讨lncRNA-BBOX1-2和FGFR1在胃癌中的表达和意义。方法:采用real-time PCR检测45例胃癌组织和相应癌旁正常组织中的lncRNABBOX1-2和FGFR1表达,分析两者表达与胃癌临床病理特征的关系。以ROC曲线分析lncRNA-BBOX1-2和FGFR1判断胃癌发生和淋巴结转移的效能。以si RNA-BBOX1-2转染人胃癌细胞株SGC-7901,real-time PCR和蛋白质印迹法检测FGFR1表达。结果:胃癌组织中的lncRNA-BBOX1-2和FGFR1表达显著高于癌旁正常组织(P <0. 05); lncRNA-BBOX1-2表达与肿瘤淋巴结转移和TNM分期呈显著正相关(P <0. 05),FGFR1表达与肿瘤分化程度、浸润深度、淋巴结转移和TNM分期呈显著正相关(P <0. 05),两者在胃癌组织中的表达则呈弱相关关系(r=0. 344,P <0. 05)。以RNA干扰技术敲除lncRNA-BBOX1-2表达后,SGC-7901细胞FGFR1 m RNA和蛋白表达显著下调(P <0. 05)。ROC曲线分析显示,lncRNA-BBOX1-2和FGFR1对于判断胃癌发生(AUC分别为0. 916和0. 862)和淋巴结转移(AUC分别为0. 720和0. 774)有一定临床应用价值。结论:Lnc RNA-BBOX1-2和FGFR1在胃癌组织中表达上调。Lnc RNA-BBOX1-2可能通过正向调节FGFR1表达参与调控胃癌的发生、发展。Background:Dysregulation of long non-coding RNAs(lncRNAs)has been found in a variety of malignancies.Previous study revealed that lncRNA-BBOX1-2 was up-regulated in gastric cancer and fibroblast growth factor receptor 1(FGFR1)might be the potential target gene of lncRNA-BBOX1-2.Aims:To investigate the expressions and significance of lncRNA-BBOX1-2 and FGFR1 in gastric cancer.Methods:Expressions of lncRNA-BBOX1-2 and FGFR1 were detected by real-time PCR in 45 cases of gastric cancer tissues and the adjacent normal tissues.The correlations of these expressions with clinicopathological features of gastric cancer were analyzed.ROC curve analysis was used to assess the performance of lncRNA-BBOX1-2 and FGFR1 in distinguishing cancerous tissue and normal tissue and predicting lymph node metastasis.After transfected with siRNA-BBOX1-2,the expression of FGFR1 in human gastric cancer cell line SGC-7901 was detected by real-time PCR and Western blotting.Results:Both lncRNA-BBOX1-2 and FGFR1 were overexpressed in gastric cancer tissues than in adjacent normal tissues(P<0.05).The expression level of lncRNA-BBOX1-2 was positively correlated with lymph node metastasis and TNM stage(P<0.05),and the expression level of FGFR1 was positively correlated with tumor differentiation,depth of invasion,lymph node metastasis and TNM stage(P<0.05).The expression levels of lncRNA-BBOX1-2 and FGFR1 demonstrated a modest correlation with each other(r=0.344,P<0.05).When lncRNA-BBOX1-2 was knocked out by RNA interference,mRNA and protein expressions of FGFR1 were significantly decreased in SGC-7901 cells(P<0.05).ROC curve analysis showed that lncRNA-BBOX1-2 and FGFR1 might be potential biomarkers for initiation(AUC:0.916 and 0.862,respectively)and lymph node metastasis(AUC:0.720 and 0.774,respectively)of gastric cancer.Conclusions:LncRNA-BBOX1-2 and FGFR1 are up-regulated in gastric cancer tissues.LncRNA-BBOX1-2 might be a positive regulator of FGFR1 and their cooperation are involved in modulating tumorigenesis and development of gastric canc

关 键 词：长链非编码RNA LncRNA-BBOX1-2 受体 成纤维细胞生长因子 1型 胃肿瘤 肿瘤分期 

分 类 号：R73[医药卫生—肿瘤]