红芪多糖和硒化红芪多糖对口腔癌细胞作用的体外实验研究  

Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro

作　　者：曾素娟 彭博[2] 程卫东[3] 卫东锋[4] 黄文燕[2] 李云阳 赵望泓 ZENG Sujuan;PENG Bo;CHENG Weidong;WEI Dong⁃feng;HUANG Wenyan;LI Yunyang;ZHAO Wanghong(Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Pediatric Dentistry,Stomatological hospital affiliat⁃ed to Guangzhou Medical University,Guangzhou 510140,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]南方医科大学南方医院口腔科,广东广州510515 [2]广州医科大学附属口腔医院儿童口腔科,广东广州510140 [3]南方医科大学中医药学院,广东广州510515 [4]中国中医科学院中医临床基础医学研究所,北京100700

出　　处：《口腔疾病防治》2019年第12期757-762,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目（81373806）;广东省中医药局科研项目（20181164）

摘　　要：目的研究红芪多糖(sedysarum polybotys saccharides,HPS)和硒化红芪多糖(selenizated hedysarum polybotyssaccharides,SE HPS)对口腔癌细胞SCC25的影响。方法取对数生长期的口腔癌细胞系SCC25,分别加入不同浓度(0、10、25、50、100、200、400μg/mL)的HPS和SE HPS,CCK8法检测细胞增殖,流式细胞术检测细胞凋亡,RT qPCR及Westernbloting观察细胞凋亡相关的指标。结果各浓度HPS和SE HPS均可抑制SCC25增殖,50μg/mLHPS和SE HPS抑制SCC25增殖的效果最强,并呈现时间依赖性,48h内抑制增殖效果明显,48h后效果达到平台期;SE HPS抑制SCC25细胞增殖的效果强于HPS(P<0.05)。流式细胞术结果显示50μg/mLHPS和SE HPS作用于SCC2548h,凋亡率分别为25.8%、30.8%,与对照组(0μg/mLHPS和SE HPS)相比差异有统计学意义(P<0.05);RT qPCR及Westernbloting结果显示50μg/mLHPS和SE HPS作用于SCC2548h,凋亡基因Fas/Fasl的mRNA与蛋白表达均上调,与对照组相比差异有统计学意义(P<0.05)。结论HPS和SE HPS均可以抑制口腔癌细胞SCC25的增殖,SE HPS效果优于HPS,可能通过Fas/Fasl途径发挥诱导凋亡作用。Objective To study the effects of hedysarum polybotys saccharides(HPS)and selenizated hedysarum polybotys saccharides(SE⁃HPS)on the oral squamous cancer cell line SCC25.Methods Different concentrations(0,10,25,50,100,200,400μg/ml)of HPS and SE⁃HPS were added to SCC25 cells in the logarithmic growth stage.Cell proliferation was detected by the CCK⁃8 method,apoptosis was detected by flow cytometry,and apoptosis⁃related index⁃es were observed by RT⁃qPCR and Western blotting.Results The concentrations of HPS and SE⁃HPS inhibited the proliferation of SCC25 cells.The inhibitory effect of 50μg/mL HPS and SE⁃HPS on the proliferation of SCC25 cells was the strongest and was time⁃dependent.The inhibition effect significantly increased within 48 h,and the effect was achieved after 48 h.At the plateau stage,SE⁃HPS inhibited the proliferation of SCC25 cells more strongly than HPS(P<0.05).The results of flow cytometry showed that 50μg/mL HPS and SE⁃HPS acted on SCC25 cells for 48 h,and the apoptotic rates were 25.8%and 30.8%respectively.Compared with the control group(0μg/mL HPS and SE⁃HPS),the difference was statistically significant(P<0.05).RT⁃qPCR and Western blotting showed that 50μg/mL HPS and SE⁃HPS acted on SCC25 cells for 48 h,and the mRNA and protein expression levels of the apoptosis gene Fas/FasL were upregulated.The difference was statistically significant(P<0.05).Conclusion Both HPS and SE⁃HPS can inhib⁃it the proliferation of SCC25 oral cancer cells,but SE⁃HPS is superior to HPS and can induce apoptosis through the Fas/Fasl pathway.

关 键 词：红芪多糖 硒化红芪多糖 口腔鳞癌细胞株SCC25 体外实验 增殖 凋亡 FAS/FASL基因 

分 类 号：R78[医药卫生—口腔医学] R739.8[医药卫生—临床医学]