Near-infrared fluorescent labeled CGRRAGGSC peptides for optical imaging of IL-11Rα in athymic mice bearing tumor xenografts  

作　　者：Tiannv Li Jin Sun Yao Hu Min Yang Haibin Shi Lijun Tang 

机构地区：[1]Department of Nuclear Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China [2]The First Clinical Medical College of Nanjing Medical University,Nanjing,Jiangsu 210029,China [3]Molecular Imaging Center,Key Laboratory of Nuclear Medicine,Ministry of Health,Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi,Jiangsu 214063,China [4]Department of Radiology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China

出　　处：《The Journal of Biomedical Research》2019年第6期391-397,共7页生物医学研究杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China (No.81202032);Key University Science Research Project of Jiangsu Province (No. 16KJB320004);Jiangsu Provincial Health and Family Planning Commission Foundation (No.Z201502);Jiangsu Provincial Health and Family Planning Research Projects (No.H2018029);Key Laboratory of Nuclear Medicine of the Ministry of Health, Jiangsu Provincial Key Laboratory of Molecular Nuclear Medicine (No.KF201501)

摘　　要：The interleukin-11(IL-11)and the IL-11 receptorα-subunit(IL-11Rα)have been demonstrated to regulate the invasion and proliferation of tumor cells.Our study intends to evaluate a noninvasive imaging of IL-11Rαexpression in breast tumors using near-infrared(NIR)fluorescent dye Cy7-labeled IL-11 mimic peptide CGRRAGGSC.This work evaluated the IL-11Rαexpression of breast tumor cells and the binding status of this peptide to IL-11Rαin vitro and in vivo by using Western blotting,immunofluorescence staining and near-infrared fluorescence imaging.Our biochemical study showed that IL-11Rαwas overexpressed in breast tumor cells(MCF-7).The cell-binding assay demonstrated specific binding of peptide CGRRAGGSC to MCF-7 cells in vitro.In vivo imaging results showed that NIR fluorescent signals of Cy7-CGRRAGGSC were selectively accumulated in tumor and metabolic organs.While in the blocking experiment,free CGRRAGGSC obviously blocked the concentration of the Cy7-CGRRAGGSC in the tumors.These results suggested that IL-11Rαmay be used as a potential target for noninvasive imaging in IL-11Rαoverexpressed tumors.Furthermore,the imaging agent of near-infrared fluorescent dye Cy7-labeled CGRRAGGSC is suitable for IL-11Rαexpression imaging study in vivo.The interleukin-11(IL-11) and the IL-11 receptor a-subunit(IL-11 Ra) have been demonstrated to regulate the invasion and proliferation of tumor cells.Our study intends to evaluate a noninvasive imaging of IL-11 Rαexpression in breast tumors using near-infrared(NIR) fluorescent dye Cy7-labeled IL-11 mimic peptide CGRRAGGSC.This work evaluated the IL-11 Rα expression of breast tumor cells and the binding status of this peptide to IL-11 Ra in vitro and in vivo by using Western blotting,immunofluorescence staining and near-infrared fluorescence imaging.Our biochemical study showed that IL-11 Rα was overexpressed in breast tumor cells(MCF-7).The cell-binding assay demonstrated specific binding of peptide CGRRAGGSC to MCF-7 cells in vitro.In vivo imaging results showed that NIR fluorescent signals of Cy7-CGRRAGGSC were selectively accumulated in tumor and metabolic organs.While in the blocking experiment,free CGRRAGGSC obviously blocked the concentration of the Cy7-CGRRAGGSC in the tumors.These results suggested that IL-11 Rα may be used as a potential target for noninvasive imaging in IL-11 Rα overexpressed tumors.Furthermore,the imaging agent of near-infrared fluorescent dye Cy7-labeled CGRRAGGSC is suitable for IL-11 Rα expression imaging study in vivo.

关 键 词：interleukin-11 receptor molecular imaging NEAR-INFRARED breast tumors 

分 类 号：R730.43[医药卫生—肿瘤]