MiR-155-5p:在肾母细胞瘤中表达下调并抑制肾母细胞瘤细胞的增殖、迁移及促进细胞凋亡  

Expression of miR-155-5p in Wilms tumor and its regulatory role in proliferation,migration and apoptosis of Wilms tumor cells in vitro

作　　者：罗鑫 董军君[1] 何兴跃 沈炼桔[1] 龙春兰[1] 刘丰[1] 刘星[1] 林涛[1] 何大维[1] 魏光辉[1] LUO Xin;DONG Junjun;HE Xingyue;SHEN Lianju;LONG Chunlan;LIU Feng;LIU Xing;LIN Tao;HE Dawei;WEI Guanghui(Department of Pediatric Urologic Surgery,Children's Hospital of Chongqing Medical University,Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering,Ministry of Education Key Laboratory of Child Development and Disorders,National Clinical Research Center for Child Health and Disorders,China International Science and Technology Cooperation Base of Child Development and)

机构地区：[1]重庆医科大学附属儿童医院泌尿外科儿童泌尿生殖发育与组织工程重点实验室儿童发育疾病研究教育部重点实验室国家儿童健康与疾病临床医学研究中心儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室

出　　处：《南方医科大学学报》2019年第12期1476-1481,共6页Journal of Southern Medical University

基　　金：重庆市技术创新与应用示范一般项目(cstc2018jscxmsyb X0091);国家临床重点专科建设项目(国卫办医函[2013]544号)

摘　　要：目的探讨miR-155-5p在肾母细胞瘤中的表达及其对肾母细胞瘤细胞增殖、迁移及凋亡能力的影响。方法收集40例肾母细胞瘤患者的肿瘤组织及其相对应的瘤旁组织,瘤旁组织作为正常对照组,应用RTq PCR检测肾母细胞瘤组织和正常瘤旁肾脏组织中miR-155-5p的表达水平;在肾母细胞瘤细胞株G401中,运用脂质体转染技术过表达及抑制miR-155-5p;采用CCK-8实验检测肾母细胞瘤细胞增殖能力的变化;划痕实验检测肾母细胞瘤细胞迁移能力的变化;流式细胞术检测肾母细胞瘤细胞凋亡能力的变化。结果RTq PCR结果显示肾母细胞瘤肿瘤组织中miR-155-5p的表达水平显著低于正常肾脏组织,且miR-155-5p的表达与肾母细胞瘤的临床分期呈负相关,差异有统计学意义(P<0.05);在人肾母细胞瘤细胞G401中上调miR-155-5p表达能够显著抑制肿瘤细胞的增殖和迁移,促进凋亡(P<0.05);下调miR-155-5p表达能够显著促进肿瘤细胞的增殖和迁移,抑制凋亡(P<0.05)。结论miR-155-5p在肾母细胞瘤患者肿瘤组织中表达显著下调,且其表达量与临床分期呈负相关;miR-155-5p可抑制肾母细胞瘤细胞增殖和迁移,促进细胞凋亡;miR-155-5p有望成为肾母细胞瘤诊断、治疗和预后评估的一个新的潜在标志物。Objective explore the expression of miR-155-5 p in Wilms tumor and its effect in regulating the proliferation,migration and apoptosis of Wilms tumor cells.Methods Specimens of tumor tissues and paired adjacent tissues were obtained from 40 patients with Wilms tumor for detection of the expression levels of miR-155-5 p using RT-q PCR.Wilms tumor cell line G401 was transfected with miR-155-5 p mimics and miR-155-5 p inhibitor to induce miR-155-5 p over-expression and its inhibition,respectively,and the changes in the cell proliferation,migration and apoptosis were assessed using cell counting kit-8(CCK-8),wound healing assay and fl ow cytometry.Results RT-q PCR showed that the expression of miR-155-5 p decreased significantly in Wilms tumor tissues as compared with normal kidney tissues and was significantly associated with TNM stage(P<0.05).In G401 cells,over-expression of miR-155-5 p significantly inhibited the cell proliferation and migration and promoted cell apoptosis(P<0.05),and down-regulation of miR-155-5 p obviously enhanced the proliferation and migration and suppressed apoptosis of the cells(P<0.05).Conclusion miR-155-5 p is down-regulated in Wilms tumor and its expression level is correlated with TNM stage.miR-155-5 p participates in the progression of Wilms tumor by inhibiting the proliferation and migration and promoting apoptosis of the tumor cells,and may serve as a novel biomarker for diagnosis,therapy and prognostic evaluation of Wilms tumor.

关 键 词：肾母细胞瘤 miR-155-5p 增殖 迁移 凋亡 

分 类 号：R73[医药卫生—肿瘤]