1株太湖流域土著溶藻菌的溶藻特性研究  

Isolation and Identification of Algae-Bacterial Bacteria WS8 and Characteristics of Microcystis aeruginosa

作　　者：张冬慧 杨苏文[1] 赵宗升[2] 金位栋[1] 闫玉红[1] ZHANG Donghui;YANG Suwen;ZHAO Zongsheng;JIN Weidong;YAN Yuhong(National Engineering Laboratory for Lake Pollution Control and Ecological Restoration,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;School of Civil and Architectural Engineering,Beijing Jiaotong University,Beijing 100044,China)

机构地区：[1]中国环境科学研究院,湖泊水污染治理与生态修复技术国家重点实验室,北京100012 [2]北京交通大学土木与建筑工程学院,北京100044

出　　处：《环境科学与技术》2019年第9期22-29,共8页Environmental Science & Technology

基　　金：国家水体污染控制与治理科技重大专项（2017ZX07204005）

摘　　要：从太湖流域宛山荡蓝藻爆发地底泥样品中分离岀1株溶解铜绿微囊藻的细菌,编号为WS8。生理生化鉴定及16S rDNA序列分析结果表明,该菌与Bacillus amyloliquefaciens (GenBank登录号为KC441776)的16S rDNA序列相似度为99%。通过液体感染法(与铜绿微囊藻共培养)考察了该菌溶藻特性及溶藻机制。结果表明,溶藻菌WS8具有显著的溶藻效果。稳定期菌液溶藻效果最强,4 d溶藻率高达91.7%;菌液溶藻效果随投加比例的增加而增强,但投加量5%与10%处理组溶藻效果无显著差异,4 d溶藻率分别为91.3%及93.3%;菌液对高浓度水华藻的抑制作用较迟滞,对低浓度水华藻的抑制作用迅速,但4 d溶藻率并无显著差异;菌液对温度及pH适应能力较强,具有较好的工程应用价值,在30℃,pH 8时溶藻率最高。溶藻菌WS8的溶藻机制可归纳为:菌株分泌具有溶藻活性的胞外活性物质,该活性物质会引起藻细胞丙二醛含量升高,对藻细胞产生氧化损伤,破坏藻细胞保护酶,从而降低藻细胞生物量。A strain of bacteria dissolving Microcystis aeruginosa was isolated from the sediment in the waters of the cyanobacteria in the Taihu Lake Basin, numbered WS8. Physiological and biochemical identification and 16 S rDNA sequence analysis showed that the strain was Bacillus sp., and the 16 S r DNA sequence similarity with Bacillus amyloliquefaciens(GenBank accession number is KC441776) was 99%. The algae-dissolving characteristics and algae-dissolving mechanism of the strain were investigated by liquid infection method(co-culture with Microcystis aeruginosa). The results showed that the algicidal bacteria WS8 had a significant inhibitory effect on Microcystis aeruginosa. In the stable period, the algae-dissolving effect of the bacterial liquid was the strongest, and the algae-dissolving rate was as high as 91.7% in 4 d. The algae-dissolving effect of the bacterial liquid increased with the increase of the dosage ratio, but there was no significant difference in the algae-dissolving effect between the 5% and 10% treatment groups. The rates were 91.3% and 93.3%, respectively. The inhibitory effect of bacterial liquid on high concentration of aquatic algae was slower, and the inhibition of low concentration of algae was rapid, but there was no significant difference in the algae rate of 4 d. It has strong pH adaptability and good engineering application value. The highest algae-dissolving rate is obtained at 30 ℃ and pH 8. The algae-dissolving mechanism of the algicidal bacteria WS8 can be summarized as follows: the strain can secrete extracellular secretions with algae-dissolving activity, and the sterile filtrate can cause algae cells to increase malondialdehyde content, oxidative damage to algae cells, and damage algae cell protection. Enzymes, thereby reducing algal cell biomass.

关 键 词：溶藻芽孢杆菌 铜绿微囊藻 溶藻特性 溶藻作用方式 

分 类 号：X172[环境科学与工程—环境科学]