过氧化氢氧化桑色素动力学荧光法测定痕量钴(Ⅱ)  

Kinetic Fluorimetric Determination of Trace Cobalt(Ⅱ) Based on Oxidation of Morin with Hydrogen Peroxide

作　　者：盛丽[1] 张萌 张晓 李海粟 薛自燕 苏碧泉[1] Sheng Li;Zhang Meng;Zhang Xiao;Li Haisu;Xue Ziyan;Su Biquan(College of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070;Key In stitute of Lightwave Technology,Beijing Jiaotong University,Beijing 100044;Zhangye Food and Drug Inspection Testing Center,Zhangye 734000)

机构地区：[1]兰州交通大学化学与生物工程学院,兰州730070 [2]北京交通大学光波技术研究所,北京100044 [3]张掖市食品药品监督管理局,张掖734000

出　　处：《化学通报》2019年第12期1134-1137,共4页Chemistry

基　　金：兰州交通大学实验教改项目(201820);中国博士后科学基金项目(2019M650467)资助

摘　　要：建立了以Co(Ⅱ)为催化剂,催化过氧化氢氧化桑色素使其荧光减弱的催化动力学荧光法。在pH 10. 70的Na2B4O7·10H2O-NaOH缓冲溶液中,痕量钴(Ⅱ)离子对0. 24%的H2O2溶液氧化4. 0×10-7mol/L的桑色素溶液有明显的催化作用。在最大吸收波长处(λex/λem=416. 5nm/556. 4nm),体系的荧光强度在一定范围内与Co(Ⅱ)的浓度呈线性相关,线性范围为0. 8~8. 0μg/L,检出限为0. 14μg/L。考察了体系的共存离子干扰情况,对灰钙土和分子筛样品进行了分析检测,回收率分别为95. 0%~110. 0%和98. 9%~101. 9%。A catalytic kinetic fluorescence method was developed using Co(Ⅱ) as a catalyst to catalyze the oxidation of morin by hydrogen peroxide. In the buffer solution of Na2 B4 O7·10 H2 O-NaOH at pH 10. 70,trace cobalt(Ⅱ) ion has obvious catalytic effect on the oxidation of 4. 0×10-7 mol/L morin solution with 0. 24% H2 O2 solution. At the maximum wavelength(λex/λem= 416. 5 nm/556. 4 nm),the fluorescence intensity of the system is linearly correlated with the concentration of Co(Ⅱ) in a concentration range of 0. 8 ~ 8. 0 μg/L,and the detection limit is0. 14 μg/L. The optimal conditions of the system were optimized and the ion interference of the system was investigated. The sierozem and molecular sieves samples were analyzed,the recovery rate were 95. 0% ~ 110. 0% and98. 9% ~ 101. 9%.

关 键 词：催化动力学荧光法 钴离子 桑色素 过氧化氢 

分 类 号：S153.6[农业科学—土壤学] O657.3[农业科学—农业基础科学] O643.12[理学—分析化学]