喉癌及癌旁组织小眼畸形相关转录因子基因表达及临床意义  

Expression and clinical significance of microphthalmia-asociated transcription factor gene in laryngeal cancer and its adjacent tissues

作　　者：王学昌 钱晓云[2] 顾亚军[2] 宋盼盼[2] 沈晓辉[2] 唐瑶 高下[1,2] WANG Xue-chang;QIAN Xiao-yun;GU Ya-jun;SONG Pan-pan;SHEN Xiao-hui;TANG Yao;GAO Xia(n.t.department,drum tower school of clinical medicine,nanjing medical university,nanjing,jiangsu 210008,china;不详)

机构地区：[1]南京医科大学鼓楼临床医学院耳鼻咽喉头颈外科,江苏南京210008 [2]南京大学医学院附属鼓楼医院耳鼻咽喉头颈外科南京鼓楼医院耳鼻咽喉研究所,江苏南京210008 [3]南京大学医学院,江苏南京210008

出　　处：《中国临床研究》2019年第12期1609-1612,共4页Chinese Journal of Clinical Research

基　　金：江苏省科教强卫工程项目资助(ZDXKB2016015);南京市卫生青年人才培养工程项目(QRX17012)~~

摘　　要：目的分析喉癌及癌旁组织中小眼畸形相关转录因子(microphthalmia-asociated transcription factor,MITF)mRNA的表达情况,推测其临床意义。方法收集2014年3月至2016年6月南京鼓楼医院耳鼻咽喉头颈外科就诊的33例患者喉癌及癌旁新鲜组织标本,提取组织RNA,采用qPCR的方法检测MITF mRNA表达情况,收集患者的临床病理资料进行统计分析。结果喉癌组织中MITF mRNA的ΔCt值(10.01±4.61)高于相应的癌旁组织(7.64±6.57),MITF在喉癌中的表达高于癌旁组织,差异有统计学意义(P=0.016)。喉癌组织中的MITF基因表达在不同年龄(P=0.259)、不同T分期(P=0.118)、是否淋巴结转移(P=1.000)、不同肿瘤部位(P=0.079)、不同分化程度(P=0.481)之间无统计学差异。但MITF mRNA在晚期(Ⅲ和Ⅳ期)喉癌患者中的表达明显高于早期(Ⅰ和Ⅱ期),差异有统计学意义(P=0.039)。33例喉癌组织中MITF低表达患者的中位总生存期为29个月(12~52个月),高表达患者的中位总生存期为30个月(8~56个月),低表达与高表达患者中位总生存期差异无统计学意义(χ^2=1.594,P=0.207)。结论喉癌组织中MITF mRNA表达较癌旁组织高,且MITF mRNA在晚期喉癌患者组织中的表达高于早期患者,MITF基因的表达可能与喉癌的发生发展相关。Objective To analyze the expression of microthromia-associated transcription factor(MITF)mRNA in laryngeal cancer and its adjacent tissues,and to speculate its clinical significance.Methods A total of 33 patients with laryngeal cancer who received treatment at Nanjing Drum Tower Hospital from March 2014 to June 2016 were selected,and their fresh specimens of laryngeal cancer and adjacent tissues were collected.Tissue RNA was extracted,expression of MITF mRNA was detected by qPCR,and clinicopathological data of patients were collected for statistical analysis.Results TheΔCt value of MITF mRNA in laryngeal carcinoma was significantly lower than that in the corresponding adjacent tissues(10.01±4.61 vs 7.64±6.57,P=0.016).There was no significant difference in the expression of MITF mRNA in different age(P=0.259),T stage(P=0.118),lymph node metastasis(P=1.000),tumor site(P=0.079),and differentiation degree(P=0.481).However,the expression of MITF mRNA in patients with stageⅢandⅣlaryngeal cancer was significantly higher than that in patients with stageⅠandⅡlaryngeal cancer(P=0.039).The median total survival time of 33 patients with low expression of MITF was 29 months(12-52 months),and that of the patients with high expression was 30 months(8-56 months),and there was no significant difference between the two groups(χ^2=1.594,P=0.207).Conclusion The expression of MITF gene in laryngeal cancer is higher than that in adjacent tissues,and the expression of MITF mRNA in advanced laryngeal cancer is higher than that in early laryngeal cancer.The expression of MITF gene may be related to the occurrence and development of laryngeal cancer.

关 键 词：喉癌 小眼畸形相关转录因子 基因表达 临床病理 

分 类 号：R739.65[医药卫生—肿瘤]