天敌声音应激对SD大鼠的焦虑样行为的影响  

Effect of predatory sound stress on the anxiety-like behavior of Sprague-Dawley rats

作　　者：陈超杰 钟志凤[2,3] 何嘉莉[1] 张水华 CHEN Chaojie;ZHONG Zhifeng;HE Jiali;ZHANG Shuihua(School of Chemical Engineering and Resource Recycling,Wuzhou University,Wuzhou 543002,China;Fujian Center for Safety Evaluation of New Drug,Fujian Medical University,Fuzhou 350122;Department of High Altitude Operational Medicine,College of High Altitude Military Medicine,Army Medical University,Chongqing 400038)

机构地区：[1]梧州学院,化学工程与资源再利用学院,广西梧州543002 [2]福建医科大学,福建省新药安全性评价中心,福州350122 [3]陆军军医大学,高原军事医学系高原作业医学教研室,重庆400038

出　　处：《中国比较医学杂志》2019年第12期1-9,共9页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(81860736);福建省自然科学基金项目(2017J01443);广西自然科学基金项目(2017GXNSFBA198060);广西高等学校千名中青年骨干教师培育计划资助项目[桂教人〔2018〕18号];福建省科技计划引导性项目(2018Y0023);广西高校大学生创新创业训练计划项目(201811354096)

摘　　要：目的观察天敌声音在不同时长和重复应激下对SD大鼠焦虑样行为的影响。方法观察大鼠经单次或重复天敌声音应激后,在旷场试验(OFT)和高架十字迷宫(EPM)的行为变化。OFT和EPM的平均速度作为大鼠整体活动水平的指标,OFT中央区和EPM开闭区的路程比例和停留时间比例用于评价大鼠焦虑状态。结果在单次应激前,各组大鼠整体活动水平一致,均处于低焦虑状态;应激后第1(OFT)、3(OFT)和7天(EPM),与空白对照组相比,10 min和60 min单次天敌声音应激均能显著降低大鼠在OFT中央区或EPM开臂区的路程比例和停留时间比例(P<0.01)。大鼠在重复天敌声音应激后第1(EPM)、3(OFT)和7天(EPM),均可见OFT中央区或EPM开臂区的路程比例和停留时间比例较空白对照组显著下降(P<0.01);同时在重复应激后第7天,地西泮(1 mg/kg,i.p.)能有效逆转天敌声音应激大鼠的这种焦虑状态水平(P<0.01)。结论天敌声音应激能增加大鼠的焦虑样行为,而且单次天敌声音应激在较短时间内(10 min)起效,作用持久(不少于7 d),重复应激不耐受。Objective To observe the effect of single and repeated predatory sound stresses on the anxiety-like behavior of Sprague-Dawley rats.Methods After single and repeated predatory sound stresses,the behaviors of rats were recorded by open-field test(OFT)and elevated plus-maze(EMP)test,respectively.The mean velocities of OFT and EPM were measured as an index of movement,and the percentages of distance traveled(D%)and persistent time spent(T%)in the center zone of OFT and open arms of the EMP,respectively,were determined as parameters of anxiety-like behavior and used to evaluate the anxiety of rats.Results Before a single stress,movement in each group of rats was similar,but showed a lower baseline during anxiety.At days 1(OFT),3(OFT)and 7(EPM)after stress,10 and 60 min treatments with a single predatory sound stress significantly decreased the D%and T% in the center zone of OFT or open arms of the EPM compared with control and white noise groups,respectively(P<0.01).Similarly,rats at days 1(EPM),3(OFT)and 7(EPM)after repeated predatory sound stresses exhibited lower D% and T% in the center zone of OFT or open arms of the EPM compared with control and white noise groups,respectively(P<0.01).At day 7 after repeated stresses,diazepam(1 mg/kg i.p.)reversed the levels of anxiety in predatory-sound-stressed rats(P<0.01).Conclusions Predatory sound stress increases the anxiety-like behavior of rats.In addition,a single predatory sound stress over a short duration(10 min)induced stress that persisted for at least 7 days,with no tolerance to repeated stresses.

关 键 词：焦虑动物模型 天敌声音 SD大鼠 旷场试验 高架十字迷宫测试 

分 类 号：R-33[医药卫生]